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The Effect Of Temperature on the Ability of Bacteriophage to Prevent Escherichia Coli Growth

Posted: Tue Jul 12, 2016 9:44 am
by deleted-365719
I'm doing an experiment like the "biowarfare" one listed on this website. However, I'm confused about the phage dilution part.
The procedure for 30 minutes before the experiment reads: https://www.sciencebuddies.org/science- ... #procedure
What is going on with the titrations?

Re: The Effect Of Temperature on the Ability of Bacteriophage to Prevent Escherichia Coli Growth

Posted: Thu Jul 14, 2016 10:52 am
by SciB
I'm not sure what your question is. The purpose of doing dilutions is so the number of phage plaques is in the countable range--100 to 300. If the number of plaques is less than 50 your results won't be accurate. If the plate has so many plaques that they merge together then they are uncountable--TMTC.

If this does not answer your question, post again and ask clearly what you want to know.

Sybee