The Effect Of Temperature on the Ability of Bacteriophage to Prevent Escherichia Coli Growth

Ask questions about projects relating to: biology, biochemistry, genomics, microbiology, molecular biology, pharmacology/toxicology, zoology, human behavior, archeology, anthropology, political science, sociology, geology, environmental science, oceanography, seismology, weather, or atmosphere.

Moderators: AmyCowen, kgudger, MadelineB, Moderators

Locked
deleted-365719
Posts: 2
Joined: Wed Jun 29, 2016 10:51 am
Occupation: Student

The Effect Of Temperature on the Ability of Bacteriophage to Prevent Escherichia Coli Growth

Post by deleted-365719 »

I'm doing an experiment like the "biowarfare" one listed on this website. However, I'm confused about the phage dilution part.
The procedure for 30 minutes before the experiment reads: https://www.sciencebuddies.org/science- ... #procedure
What is going on with the titrations?
SciB
Expert
Posts: 2071
Joined: Fri Feb 01, 2013 7:00 am
Occupation: Retired molecular biologist, university researcher and teacher
Project Question: I wish to join Scibuddies to be able to help students achieve the best science project possible and to understand the science behind it.
Project Due Date: n/a
Project Status: Not applicable

Re: The Effect Of Temperature on the Ability of Bacteriophage to Prevent Escherichia Coli Growth

Post by SciB »

I'm not sure what your question is. The purpose of doing dilutions is so the number of phage plaques is in the countable range--100 to 300. If the number of plaques is less than 50 your results won't be accurate. If the plate has so many plaques that they merge together then they are uncountable--TMTC.

If this does not answer your question, post again and ask clearly what you want to know.

Sybee
Locked

Return to “Grades 9-12: Life, Earth, and Social Sciences”