Measuring bacteria growth--help!

[punkette]

http://www.cat.cc.md.us/courses/bio141/ ... insol.html
^^According to that photo, Serratia marcescens looks like that after cultivated on a petri plate. Then how am I to "measure" its growth? It doesn't seem like I can count the bacterial colonies? Or could I? Any informationwill be highly appreciated!

[shijun]

Hi Punkette,

You are in luck. I've consulted a friend of mine who is an expert in this area, and this is what she has to say:

-QUOTE-
If anyone tried to do an experiment measuring growth they wouldn't ever
have streaked it out like that...the only thing that plate's useful for is
to take cells to start cultures with. To measure growth/viability he should
take a toothpick headful, put it in a few mLs media, dilute it about 1000
fold, then replate a few hundred microliters on a fresh plate. Or if he were
looking at growth rate he should just grow it in some media, take aliquots,
and measure optical density over time.
-END QUOTE-

I hope this helps!

[JULIE ANN BONSUBRE]

hi,

why not try Bauer-Kirby Disk Diffusion....or try... http://whitewolf.newcastle.edu.au/techi ... ounts.html...

i hope it did help.

good luck.



julie

[JULIE ANN BONSUBRE]

but the susceptivility test is usually used to measure the growth of microorganism in its response to some chemical...