In Simpler Words Please....

[eternal_corrector]

Hey if anyone can convert this part of an article I found in the Project Ideas area in simpler words so I can comprehend what it means:

(The degree of inhibition for each bacterium and each disinfectant concentration was calculated using viable cell count method. Resistance was measured by sequential change in the zones of inhibition and percent inhibition of the bacteria exposed to the specific disinfectant.)


Please and thank you!!!

[deleted-71490]

External_corrector:

An explanation would be as follows -
A known number of bacterial cells like 1 X 10^8 were treated with a known volume of disinfectant at different concentrations.

The disinfectant treatments could be Lysol, Purex or rubbing alcohol.
When the different treatments are added at the same volume, the relative number of cells will be the same for each treatment. Start with 100 cells/ml and dilute by 1/10 you then have 10 cells/ml.

After treatment serial dilutions were made of the treatments (1/10, 1/00, 1/000 and so on). A known volume of the dilutions was plated out and the cells allowed to grow. A count of the colonies growing on the plate times the dilution factor will give the number of cells/ml after tretament. For example -You started with 1 X 10^8 (100,000,000) cells, disinfectant treatment diluted the number 1/00, giving 1 X 10^7 (10,000,000) at treatmnet. After an exposure time to the disinfectants, the serial dilutions were made. Lets say there were 1 colony on the plate diluted 1 X 10^ 5 for Lysol. That means for that treatment the bacterial cell count went from 10,000,000 to 100,000.

When you write about inhibition zones and bacteria you usually are describing something like this -
An agar plate is flooded with a bacterial suspension. A paper disk soaked in a test solution is then placed in the center of the plate. If the test solution inhibits growth of bacteria a clear zone (no bacterial growth) occurs around the paper disk. The percent inhibition is calculated by dividing the distince in mm from the edge of the disk to the bacterial growth by the distance from the edge of the disk to the edge of the Petri plate.

I hope this helps

Matt Mulanax