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What is the concentration used for the liquid bacterial suspension? Is it a light or heavy suspension?
What media do you recommend for the suspension?
Does is E. Coli K-12 has another name and/or number? The lab where I'm doing this uses E. Coli atcc 25922
When you expose the plate to the UV light does the lid have to be on the plate or not?
Death Rays: What Duration of Ultraviolet Exposure Kills bact
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Bryan08
- Posts: 1
- Joined: Sun Sep 28, 2008 12:26 pm
- Occupation: Student
- Project Question: Death Rays: What Duration of Ultraviolet Exposure Kills Bacteria? for this project what is the concentration of the liquid bacterial suspension and E. Coli K-12 has another name and/or number?
- Project Due Date: Novemember 1, 2008
- Project Status: I am just starting
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deleted-71827
- Former Expert
- Posts: 404
- Joined: Tue Sep 18, 2007 3:27 pm
- Occupation: Research Assistant
- Project Question: Neuroregeneration
- Project Due Date: N/A
- Project Status: Not applicable
Re: Death Rays: What Duration of Ultraviolet Exposure Kills bact
Hi Bryan,
This is a really interesting idea for a science project. I think that the liquid bacterial suspension is basically the solution that holds all the bacteria in it (e.coli) so the e. coli is already in this suspension. Here is a link of the other names of e. coli K12...but I am not quite sure where your particular strain falls.
http://www.uniprot.org/taxonomy/83333
Hope this helped, good luck!
This is a really interesting idea for a science project. I think that the liquid bacterial suspension is basically the solution that holds all the bacteria in it (e.coli) so the e. coli is already in this suspension. Here is a link of the other names of e. coli K12...but I am not quite sure where your particular strain falls.
http://www.uniprot.org/taxonomy/83333
Hope this helped, good luck!
"There is a single light of science, and to brighten it anywhere is to brighten it everywhere." -Isaac Asimov
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ScienceExpert123
- Former Expert
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- Joined: Sat Sep 20, 2008 9:26 am
- Occupation: Student
- Project Question: n/a
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Re: Death Rays: What Duration of Ultraviolet Exposure Kills bact
Dear Bryan,
I think it would be a good idea to ask someone in the lab that you are doing the experiment in (like a teacher, scientist, or lab technician) about the concentrations and type of media used. I know from experience that certain labs use different media and concentrations than other labs because they have found from experience that these types of media and concentrations work best. I have done tissue culture in the past and I know that a widely used media is DMEM (Dulbecco's Modified Eagle Medium), which you may want to put into a search engine; although, this media is mostly used with cell culture, I know that bacteria will grow in it (if you buy this media make sure that you buy it anti-biotic free). Also, I think that the UV rays will penetrate the bacteria through the plate or petri dish with the cover on it.
Also, I want to remind you that bacteria, especially certain types of e. coli, are very dangerous and it might be best that, for your safety, you let an adult who is working in the lab (teacher, scientist, or lab technician) to plate, suspend, replace the media, and work with the bacteria. If you do work with the bacteria make sure that you always wear gloves and clothing that covers up your skin. If your clothing or skin comes in contact with the bacteria make sure to wash off the clothing/skin and you might want to put a little ethanol on it (ethanol kills bacteria, but won't harm you when you put it on your skin (DO NOT SWALLOW ETHANOL or put it in your eye, ect.)). If you come in contact with bacteria make sure you contact an adult in the lab right away and ask them what to do.
I hope this helped. Good luck.
I think it would be a good idea to ask someone in the lab that you are doing the experiment in (like a teacher, scientist, or lab technician) about the concentrations and type of media used. I know from experience that certain labs use different media and concentrations than other labs because they have found from experience that these types of media and concentrations work best. I have done tissue culture in the past and I know that a widely used media is DMEM (Dulbecco's Modified Eagle Medium), which you may want to put into a search engine; although, this media is mostly used with cell culture, I know that bacteria will grow in it (if you buy this media make sure that you buy it anti-biotic free). Also, I think that the UV rays will penetrate the bacteria through the plate or petri dish with the cover on it.
Also, I want to remind you that bacteria, especially certain types of e. coli, are very dangerous and it might be best that, for your safety, you let an adult who is working in the lab (teacher, scientist, or lab technician) to plate, suspend, replace the media, and work with the bacteria. If you do work with the bacteria make sure that you always wear gloves and clothing that covers up your skin. If your clothing or skin comes in contact with the bacteria make sure to wash off the clothing/skin and you might want to put a little ethanol on it (ethanol kills bacteria, but won't harm you when you put it on your skin (DO NOT SWALLOW ETHANOL or put it in your eye, ect.)). If you come in contact with bacteria make sure you contact an adult in the lab right away and ask them what to do.
I hope this helped. Good luck.