DNA Sequencing - Fluorescent Dye

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juman
Posts: 10
Joined: Fri Nov 13, 2009 10:55 am
Occupation: Student
Project Question: Where is plasmid DNA found in the cell?
Why do some people sequence plasmid DNA and chromosomal DNA?
What is the difference between lysate and bacterial lysate?
Project Due Date: Nov 15, 2009
Project Status: I am just starting

DNA Sequencing - Fluorescent Dye

Post by juman »

Hi,

A part of my science fair project talks about the Dye-terminator sequencing. And how this mehtod uses nucleotides that have been given a fluorescent colour.
I am wondering how that is done, how do nucleotides get a fluorscent dye?

Thank you. Please reply as soon as possible.
deleted-71417
Former Expert
Posts: 932
Joined: Wed Oct 03, 2007 12:24 am

Re: DNA Sequencing - Fluorescent Dye

Post by deleted-71417 »

Hi,

Here is an explanation of the sequenceing techniques:

http://en.wikipedia.org/wiki/DNA_sequencing

This book exerpt from Google also describes the technique:

http://books.google.com/books?id=X5yNv0 ... ng&f=false

Here is an explanation in more of an outline form:

http://74.125.155.132/search?q=cache:7W ... clnk&gl=us

I hope this answers the question. This is an important subject of very practical importance in life sciences today. Good luck with the project!

Best regards,

Barrett L. Tomlinson
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