Bioluminescence project: opaque boxes

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deleted-32471
Posts: 8
Joined: Sun Oct 11, 2009 3:21 pm
Occupation: student
Project Question: I am doing a science project on bacterial transformation using the antibiotic ampicillin and I want to extend it further with research. To do this experiment, I need guidance and a lab to perform dna extraction from bacteria and insertion to it with ampicillin. Can you recommend a specific research lab that will help me? Can you also recommend me with mild and harmless types of bacteria for my experiment?
Project Due Date: January 10, 2010
I would like to finish the experiment much before the deadline so I can extend it further.
Project Status: I am conducting my research

Bioluminescence project: opaque boxes

Post by deleted-32471 »

My project on the bioluminescence of marine plankton requires 3 opaque boxes to be put under a light for long periods of time. Do metal boxes work? I am just worried if metal boxes heat up and affect the temperature. Can you help me ?
deleted-71791
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Posts: 11
Joined: Tue Nov 24, 2009 12:14 pm
Occupation: University Student
Project Question: N/A
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Project Status: Not applicable

Re: Bioluminescence project: opaque boxes

Post by deleted-71791 »

Hi,

What you could do is place the plankton in glass containers or plastic cups and then take standard cardboard boxes, paint the inside white so that the light doesn't get absorbed and place the glass containers or plastic cups with the plankton inside of the boxes. Place a black fabric cloth over the top of the box and place the light inside and make sure the boxes are in a dark storage space.

While this is for dinoflagellates, these links may be of interest to you: http://jrscience.wcp.muohio.edu/FieldCo ... rBioA.html

Bioluminescence: Investigating Glow-in-the-Dark Dinoflagellates https://www.sciencebuddies.org/science- ... p033.shtml

Hope this helps. Good luck with your project!
carolinethorn
Former Expert
Posts: 393
Joined: Tue Sep 20, 2005 2:40 pm

Re: Bioluminescence project: opaque boxes

Post by carolinethorn »

I think your question is a good one. I too would be worried that metal boxes would heat up and increase the temperature.

The other expert has some good suggestions for alternatives to metal boxes.

Best of luck,
Caroline
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