growing yeast on agar plates

[deleted-61955]

I'm just getting started on a project to look at the effects of light, moisture and a commercial ear cleansing preparation on the growth of yeast in the ears of spaniel dogs. I've got nutrient agar plates set up and ready to innoculate. How long should I expect ot wait before I have good growth to begin the manipulation of variables?

[deleted-71827]

Hi,
Usually it takes a couple days to get the yeast growing well on the agar plates before you can make the transfer. The best way is to check the plates and see if there is a decent amount of growth. You should have enough growth so that you have enough yeast to test them with the different variables and monitor their growth. If you need some help interpreting your plates, you can always check out the ScienceBuddies guide here-

https://www.sciencebuddies.org/science- ... ates.shtml

Hope this helps, good luck!

[donnahardy2]

Hi,

This is a very interesting project. One of the challenges here is how to measure results. Here is a website that describes how to measure antibiotic resistance. You might be able to adapt your project to this technique:

http://www.hhmi.org/biointeractive/dise ... vities.pdf


It is interesting, but I had some difficulty finding scientific references on this subject. I’m sure the subject has been studied, so you should try to do more background reading from the scientific literature to learn more about your topic. Here is one reference, which includes a bibliography of other references that might help you. :

http://www.scielo.br/scielo.php?pid=S15 ... ci_arttext


I did find a couple of non-scientific websites that suggest that chronic yeast infections are due to food allergies:

http://www.peteducation.com/article.cfm ... 82&aid=143

http://www.dog-health-guide.org/yeastin ... ogear.html

This one is interesting; it looks like scientific references, but it was not published in a peer-reviewed scientific journal. But the information is interesting and probably worth investigating further.

http://www.dermapet.com/articles/art-03.html

So this is definitely a topic that needs more valid scientific research. Your project is definitely worthwhile pursuing.


Donna Hardy