k

[nsaladino12]

k

[donnahardy2]

Hi,

This is a very interesting topic. Are you doing this project?

https://www.sciencebuddies.org/science- ... p012.shtml

Or this one?

https://www.sciencebuddies.org/science- ... p013.shtml

The incubation temperature depends on the bacteria you are growing. Most likely, the bacteria will grow faster if you can use a temperature between 30 and 35 degrees Centigrade. If you do not have a temperature controlled incubator, then inoculate the plates, and turn them upside down and then put them in a warm place on top of the water heater, for example, after you verify the temperature is not higher than 42 degrees Centigrade, which would be too high. If you leave them at room temperature, the bacteria will grow slowly, and it could take 2-3 days before you see visible colonies.

Here is some helpful information on agar plates from the Science Buddies website:

https://www.sciencebuddies.org/science- ... tion.shtml

https://www.sciencebuddies.org/science- ... ques.shtml

I’m not sure exactly how you are setting up your plates, and the source of the bacteria, so if you want to post your protocol, I would be happy to make comments that would help ensure your success.

Donna Ha

[nsaladino12]

k