Glucose and cell volume

[Tiera]

What is the effect of glucose on cell volume?

[deleted-71828]

Hi Tiera,

Cell volume will be determined by dynamic equilibrium. A cell has a semi-permeable memrane and soutes like glucose will have an effect on cell volume (by osmosis).

You will have to consider the environment the cell is in.

Here are some links (they should point you in the right direction).

http://biology.arizona.edu/sciconn/less ... ading.html

http://biology.arizona.edu/sciconn/less ... dyn_eq.htm

http://www.emc.maricopa.edu/faculty/far ... ransp.html

http://biology.arizona.edu/sciconn/less ... ading.html

http://faculty.stcc.edu/AandP/AP/AP1pag ... lectiv.htm

I hope that helps!

[Tiera]

Thank You

[Tiera]

How Should I Test Out My Project: The Effect Of Glucose On Cell Vollume, I Need Help ASAP I Only have Until November 29th Thanks

[donnahardy2]

Hi Tiera,

It’s better to post an answer in the same topic so the experts who are following your topic can respond. Phonghuynh posted some links that provided background information and one possible experiment. You could do that experiment; otherwise you would need solutions of different concentrations of glucose, some cells to observe, and a microscope with a metric ruler. You should have a hypothesis before you start your experiment.

You do have a very short amount of time before your project is due, so let us know what resources you have available.

Donna Hardy

[Tiera]

Okay I'll Try

[Tiera]

OMG.! I Get It Now Well Cell Volume Is Dependent on the dynamic equlibrium.This is also part of the process diffusion. It Spreads areas of high concetration to areas of low concetration. Now Osmosis steps in.. Osmosis is the diffusion of water...Now diffusion is to basically equal out the molecules. To equal out these molecules it has to be some type of solute because of concentration gradient, molecules will always move down the lesser concdetration,with that in mind in this projct my solute is glucose and which ever side of the membrane that the glucose is more concentrated the molecules will start to move on the side with the lesser concentration to equal it out -equilibrium-. With a sum of all that... the effect of glucose on cell volume is that glucose (being a solute) it equals out the cell volume.
Now that i know this is need a quick way to test this. I Know I'm sort of late but I really didn't have any help and when I found this site i was very greatful. My Next question is what would me the quickest way to test this.?

[Tiera]

Oh and also for this all to happen the cell has to be a permeable cell <3

[donnahardy2]

Hi Tiera,

Do you have access to a microscope with a micrometer at school?

Donna

[donnahardy2]

Hi Tiera,

I think you understand the science behind your project. It’s really important to explain the scientific principles in the background information of your project.

Here’s an idea for a glucose osmosis experiment that uses the semi-permeable membrane of an egg. You would not need a microscope for this experiment. You would need a way to measure the change in volume of the shell-less egg in different concentrations of glucose?

http://c-lab.co.uk/default.aspx?id=9&projectid=56

Here is an osmosis experiment using dialysis tubing or a potato:

http://intranet.landmark.edu/ctolman/osmosis.htm

Here’s the information from the Science Buddies website on your project board. I recommend that you start writing the sections for your board while you are conducting your experiment.

https://www.sciencebuddies.org/science- ... oard.shtml

Does this help?


Donna Hardy

[Tiera]

THANK YOU.! It helps alot. I'm going with the first project. Now to measure the volume of the egg I could classify the egg as a sphere I guess and measure the volume of both sphers (the eggs) before and when it's over I can measure them after then graph the results would that be fine.?

[donnahardy2]

Hi,

An egg is an ovoid when it’s in the shell and there’s a different formula for calculating the volume. Here is a paper on the topic of measuring the volume of an egg.

http://ps.fass.org/content/84/3/482.full.pdf

Here is a simpler version of the calculation, although this version is in cubic inches. Since this is a science fair project, you should use metric measurements and report results in square centimeters.

http://www.ehow.com/how_7892704_calcula ... e-egg.html

Since you will be working with shell-less eggs, I don’t think you will be able to pick them up and measure them with a ruler or calipers. You will probably have to use a displacement method as described in this Wikepedia article:

http://en.wikipedia.org/wiki/Displacement_(fluid)

Since measurement is a very important part of a science fair project, you can distinguish your project by your meticulous attention to the measurement of the volume. I recommend that you do a pilot experiment with one egg to gain experience in taking your measurements. You can then make any modifications you need for you final experiment.

How many eggs for each concentration of glucose were you planning to use? I would recommend using at least 2 eggs, and if you can do 3 eggs, you will be able to do some additional statistical calculations that will enhance your project. Let me know if you need more information on this.

Also, glucose is not commonly available, so you can use sucrose, or table sugar, as an alternative for your independent variable.

Donna Hardy