biochemistry

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cutiepii101
Posts: 1
Joined: Sun Dec 11, 2011 6:08 pm
Occupation: student 9th grade
Project Question: my gel in the gel electrophoresis won't get solid. how do i make it solid?
Project Due Date: 12/13/11
Project Status: I am conducting my experiment

biochemistry

Post by cutiepii101 »

im doing gel electrophoresis and my gel wont turn solid. what do i do now???????????????????????????????????
VSegarra
Former Expert
Posts: 56
Joined: Sun Nov 06, 2011 6:48 am
Occupation: Scientist
Project Question: n/a
Project Due Date: n/a
Project Status: Not applicable

Re: biochemistry

Post by VSegarra »

Hello!
What type of gel are you running? Have you double-checked you are adding the correct amount of reagents to the gel mix?
Veronica
djschlesinger
Former Expert
Posts: 19
Joined: Fri May 27, 2011 1:00 pm
Occupation: Director of Research Genomics
Project Question: n/a
Project Due Date: n/a
Project Status: Not applicable

Re: biochemistry

Post by djschlesinger »

Hello cutiepii101,

I would also check to see if the kit you are using (or the individual ingredients) have an expiration date. Agarose (the main component of the gel you are running) can and does go bad when it gets old.

-Dave
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