Oil Eating Microbes

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caikins
Posts: 1
Joined: Mon Oct 22, 2012 10:31 am
Occupation: student: 7th grade
Project Question: For PJAS I want to do an experiment on the effectiveness of oil eating bacteria at different temperatures and PH's. Can you recommend a supplier of alcanivorax borkumenisis? I only found one( ATCC) and it was expensive! Can I use pseudomonas? If I can't get crude oil, what other types of oil would be the closest to crude oil I am going to measure the weight which should decrease due to the microbial respiration. How could I measure the CO2 given off in the respiration? Thanks!
Project Due Date: October 31, 2012
Project Status: I am conducting my research

Oil Eating Microbes

Post by caikins »

For PJAS I want to do an experiment on the effectiveness of oil eating bacteria at diff temp and pH's. Can you recommend a supplier of alcanivorax borkemenisis? I only found one (ATCC) and it was expensive! Can I use psuedomonas? What type of oil would be closest to crude oil? I am going to measure the weight which should decrease due to microbial respiration. How can I measure CO2 given off? Thanks!
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klhjbh62604
Posts: 22
Joined: Thu Sep 06, 2012 9:37 am
Occupation: Scientist
Project Question: Ask the Expert Volunteer.
Project Due Date: n/a
Project Status: Not applicable

Re: Oil Eating Microbes

Post by klhjbh62604 »

Hello:

This sounds like a great experiment. I would recommend looking at this pdf to help guide you in an experimental set-up.
http://www.powayusd.com/online/.../Oil% ... crobes.pdf

As seen in the above PDF, psuedomonas can be used for this experiment. If looking for inexpensive kits you may want to look at DNA Depot LLC.
http://www.dnadepot.com/products/kits/302.html#prod

Please let me know if you have any questions.
Kati
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