Chromatography Experiment

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Megan Hayden
Posts: 2
Joined: Fri Mar 22, 2013 3:31 am
Occupation: student 11th grade
Project Question: To determine whether there is a link between the blue coloring in M&M's and hyperactivity
Project Due Date: 25/03/2013
Project Status: I am just starting

Chromatography Experiment

Post by Megan Hayden »

For a science project, I am using Chromatography to compare the food coloring (E133) with the blue coloring in M&M's and Smarties. At the moment I have done the actual chromatography for the E133 dye as well as the blue dye in the M&M's. I was wondering how you would compare the two chromatography papers, to find out whether the m&m does contain the E133 dye. :)
Thanks :D
donnahardy2
Former Expert
Posts: 2671
Joined: Mon Nov 14, 2005 12:45 pm

Re: Chromatography Experiment

Post by donnahardy2 »

Hi Megan,

I think you are doing a variation of this project from the Science Buddies website:

https://www.sciencebuddies.org/science- ... background

If you have run food colors with known dyes with paper chromatography under identical conditions and want to know if the candies contain the same dye, then you should calculate the Rf values are described in the background information tab of the project guide.

If the dye is the same color and has the same Rf value, then you can say that you have probably identified the dye. If the dye is the same color and has a different Rf value, then you cannot conclude that you have identified the specific dye.

With either result, your science project will be a success if you can explain the science behind your project and make conclusions that match your results.

Please post again in this topic if you have more questions.


Donna Hardy
Megan Hayden
Posts: 2
Joined: Fri Mar 22, 2013 3:31 am
Occupation: student 11th grade
Project Question: To determine whether there is a link between the blue coloring in M&M's and hyperactivity
Project Due Date: 25/03/2013
Project Status: I am just starting

Re: Chromatography Experiment

Post by Megan Hayden »

Thank you so much Donna! Appreciate the feedback. I will calculate the Rf values :D
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