Antioxidants and Lung cancer

Ask questions about projects relating to: biology, biochemistry, genomics, microbiology, molecular biology, pharmacology/toxicology, zoology, human behavior, archeology, anthropology, political science, sociology, geology, environmental science, oceanography, seismology, weather, or atmosphere.

Moderators: AmyCowen, kgudger, MadelineB, Moderators

Locked
michelle409
Posts: 6
Joined: Tue Jul 09, 2013 9:31 am
Occupation: Student:11th grade
Project Question: The effects of different antioxidants in combating in vitro lipid peroxidation
Project Due Date: December 12, 2013
Project Status: I am just starting

Re: Antioxidants and Lung cancer

Post by michelle409 »

Hi,

I am trying to do a similar project, but I am planning on extracting antioxidants from a type of algae called Dunaliella and observing its effects on yeast treated with different oxidants. I was just wondering how you induced the oxidants into the cancer tissue and how you measured the oxidative stress. I am planning to measure the effects with a hemocytometer.

Thank you
Top
LuciaHuo
Posts: 16
Joined: Sat Nov 03, 2012 10:35 pm
Occupation: Student: 9th grade
Project Question: Find the affect of one type of antioxidant on one type of lung cancer tumor cell. Groups of the cells will be each injected with a different substance to increase the amount of it in the cell. Dose-response to antioxidants will be tested and comparisons will be made.
Questions: How can I increase the amount of a substance in a cell? Does the amount of a substance in one's body reflect the amount of it in one's cell? What are better procedures for this experiment?
Project Due Date: The proposal and a mentor must be found before Nov.16 of 2012 and the experiment must be done before Feb.15 of 2013.
Project Status: I am conducting my research

Re: Antioxidants and Lung cancer

Post by LuciaHuo »

Hi, I did not induce the oxidants into the cancer cells. Cancer cells generate reactive oxygen species as a part of normal cellular functions; it also generates more ROS than normal cells due to it's increased activeness.
Top
LuciaHuo
Posts: 16
Joined: Sat Nov 03, 2012 10:35 pm
Occupation: Student: 9th grade
Project Question: Find the affect of one type of antioxidant on one type of lung cancer tumor cell. Groups of the cells will be each injected with a different substance to increase the amount of it in the cell. Dose-response to antioxidants will be tested and comparisons will be made.
Questions: How can I increase the amount of a substance in a cell? Does the amount of a substance in one's body reflect the amount of it in one's cell? What are better procedures for this experiment?
Project Due Date: The proposal and a mentor must be found before Nov.16 of 2012 and the experiment must be done before Feb.15 of 2013.
Project Status: I am conducting my research

Re: Antioxidants and Lung cancer

Post by LuciaHuo »

Also, I measured ROS generation using a flow cytometer with a fluorescent probe.
Top
Locked

Return to “Grades 9-12: Life, Earth, and Social Sciences”