Autophagy Regulation in Yeast

beachbum · Post by **beachbum** » Thu Feb 09, 2006 1:48 pm

I have conducted an experiment to determine if chemical(x) regulates autophagy in yeast. However, to do my experiment I tested it under 6 different strains and 6 different dilutions, and I used 2 plates (one the experimental group and one the control) therefore, on one plate, I have 36 colonies are completely different, instead of one whole petri dish with the same strain under the same dilution. The project worked, however, when comparing the experimental group with the control, some colonies that came out significant and some didn't (verified by a t-test) such as 34/36 colonies were significant. My question is, when I state my conclusion, would I have to restate my hypothesis for every single strain under every dilution, or is there another way to reject the null hypothesis.

carolinethorn · Post by **carolinethorn** » Sun Feb 12, 2006 3:53 pm

Hi,

I am having some difficulty understanding quite what you were testing. Are you comparing the size of the colonies or just scoring yes/no if they grew?
i am also unclear how you could have different dilutions of chemical with the same plate. I also don't see any mention on how many replicates you did?

perhaps if you post some more details of your methods people could help more with the analysis.

best of luck,
Caroline