My 3rd grader and I have followed the protocol kindly suggested here https://www.sciencebuddies.org/science- ... shtml#help. We did one attempt where we crushed the strawberries very well in the plastic bag, and then added only water (no salt or detergent)- as a "negative control" for the extraction. In our "negative control" we could still see a whitish precipitate when we added alcohol- is it likely that this was also DNA, and that we had mechanically lysed enough cells to get this result without adding salt and detergent?
Thank you for any insight!
strawberry DNA question
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smbla
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- Project Question: Strawberry DNA extraction
- Project Due Date: February 23rd 2015
- Project Status: I am finished with my experiment and analyzing the data
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deleted-141593
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Re: strawberry DNA question
It's possible. It's also possible that it is salt or sugar from the strawberries themselves. Does it look the same as the precipitate you get with salt/detergent?
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smbla
- Posts: 2
- Joined: Mon Feb 16, 2015 8:34 pm
- Occupation: graduate student (mother)
- Project Question: Strawberry DNA extraction
- Project Due Date: February 23rd 2015
- Project Status: I am finished with my experiment and analyzing the data
Re: strawberry DNA question
Thank you for your reply!
It really looked like DNA, it was white and "cottony", and we could spoil it on to a skewer when we added alcohol. When we used salt and detergent there was more "cotton" formed, but this also had a hint of pink color (guessing it was pretty contaminated).
It really looked like DNA, it was white and "cottony", and we could spoil it on to a skewer when we added alcohol. When we used salt and detergent there was more "cotton" formed, but this also had a hint of pink color (guessing it was pretty contaminated).
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deleted-141593
- Former Expert
- Posts: 159
- Joined: Wed Aug 28, 2013 7:55 pm
- Occupation: Immunologist
- Project Question: Volunteer expert.
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Re: strawberry DNA question
So, given that there was less DNA-like material in the absence of salt/detergent could your child come up with a hypothesis to explain this? This would be a good exercise and a point to include in the interpretation of the results.
Cheers,
Colin
Cheers,
Colin

