strawberry DNA question

Ask questions about projects relating to: biology, biochemistry, genomics, microbiology, molecular biology, pharmacology/toxicology, zoology, human behavior, archeology, anthropology, political science, sociology, geology, environmental science, oceanography, seismology, weather, or atmosphere.

Moderators: AmyCowen, kgudger, bfinio, MadelineB, Moderators

Locked
smbla
Posts: 2
Joined: Mon Feb 16, 2015 8:34 pm
Occupation: graduate student (mother)
Project Question: Strawberry DNA extraction
Project Due Date: February 23rd 2015
Project Status: I am finished with my experiment and analyzing the data

strawberry DNA question

Post by smbla »

My 3rd grader and I have followed the protocol kindly suggested here https://www.sciencebuddies.org/science- ... shtml#help. We did one attempt where we crushed the strawberries very well in the plastic bag, and then added only water (no salt or detergent)- as a "negative control" for the extraction. In our "negative control" we could still see a whitish precipitate when we added alcohol- is it likely that this was also DNA, and that we had mechanically lysed enough cells to get this result without adding salt and detergent?
Thank you for any insight!
deleted-141593
Former Expert
Posts: 159
Joined: Wed Aug 28, 2013 7:55 pm
Occupation: Immunologist
Project Question: Volunteer expert.
Project Due Date: n/a
Project Status: Not applicable

Re: strawberry DNA question

Post by deleted-141593 »

It's possible. It's also possible that it is salt or sugar from the strawberries themselves. Does it look the same as the precipitate you get with salt/detergent?
smbla
Posts: 2
Joined: Mon Feb 16, 2015 8:34 pm
Occupation: graduate student (mother)
Project Question: Strawberry DNA extraction
Project Due Date: February 23rd 2015
Project Status: I am finished with my experiment and analyzing the data

Re: strawberry DNA question

Post by smbla »

Thank you for your reply!
It really looked like DNA, it was white and "cottony", and we could spoil it on to a skewer when we added alcohol. When we used salt and detergent there was more "cotton" formed, but this also had a hint of pink color (guessing it was pretty contaminated).
deleted-141593
Former Expert
Posts: 159
Joined: Wed Aug 28, 2013 7:55 pm
Occupation: Immunologist
Project Question: Volunteer expert.
Project Due Date: n/a
Project Status: Not applicable

Re: strawberry DNA question

Post by deleted-141593 »

So, given that there was less DNA-like material in the absence of salt/detergent could your child come up with a hypothesis to explain this? This would be a good exercise and a point to include in the interpretation of the results.

Cheers,
Colin
Locked

Return to “Grades K-5: Life, Earth, and Social Sciences”