separating proteins project

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deleted-308156
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separating proteins project

Post by deleted-308156 »

I need help finding some independent and dependent variables for a project titled "Sizing It Up! How Scientists Separate Proteins". I found it on this website,here is the link:
https://www.sciencebuddies.org/science- ... p035.shtml
I am doing this project for my 8th grade science project.
norman40
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Re: separating proteins project

Post by norman40 »

Hi cassie330,

An independent variable is something that you change in your experiment to observe a response in a dependent variable. In other words, the changes you see or measure for a dependent variable result from changes you made in an independent variable.

There’s more information and examples about variables here:

https://www.sciencebuddies.org/science- ... evariables

In the protein separation project you observe the color and UV sensitivity of solutions after chromatography. The color and UV sensitivity indicate which of three proteins are in the solutions. So the color (or UV sensitivity) of the solutions is the dependent variable.

A specific mixture of three proteins is used in the chromatography experiment. This mixture will result in a certain array of solutions after chromatography. In other words the order of elution of the proteins during chromatography is set by which proteins are included in the mixture. Because the mixture defines the solutions collected in the experiment the independent variable is the protein mixture.

I hope this helps. Please post again if you have more questions.

A. Norman
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