preparation of phosphate buffer

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ambreen
Posts: 4
Joined: Wed Jul 12, 2006 3:07 am

preparation of phosphate buffer

Post by ambreen »

Please can u tell me how to prepare a phosphate buffer of ph 7.4 using MnCl2,MgCl2,KH2PO4,K2HPO4
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Lise Byrd
Former Expert
Posts: 95
Joined: Sun Sep 18, 2005 10:00 pm

Post by Lise Byrd »

According to http://home.planet.nl/~skok/techniques/ ... ffers.html, you can create a 100-mL buffer of pH 7.4 with 77.4 mL of 0.1 M K2HPO4 and 22.6 mL of 0.1 M KH2PO4.
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