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cells
Moderators: AmyCowen, kgudger, MadelineB, Moderators
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deleted-457439
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cells
hi i had a question about let say i were to remove cheek cells from the outer layer how long will they live?
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SciB
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Re: cells
It depends on what you do with them after you remove them. Normally they don't live very long. Once out of the body, they dry out and die. Even in the mouth the cheek cells live only a few days before being replaced by new ones. There are ways to culture cells in a Petri dish but these cells have to be 'immortalized' so that they continue dividing and don't stop. That's why most of the cells that we grow in tissue culture are tumor cells.
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SciB
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Re: cells
Sorry, but cell culturing is a pretty complicated job that you HAVE to do in a lab. Check Youtube videos that show methods of cell culture and you will seen what mean. It's not difficult. It just requires expensive equipment and reagents and the knowledge of how to use them. You could do it if you had a lab to work in and someone to take the time to show you the methods and supervise your technique.
Please post again if you have more questions.
Sybee
Please post again if you have more questions.
Sybee
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deleted-457439
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Re: cells
thank you for the help, about around how expensive do you think It would be for basic culturing? I already have a microscope and a petri dish.
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SciB
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Re: cells
Take a look at this Youtube video on culturing mammalian cells: https://www.youtube.com/watch?v=kbhQ29vRqs4
If you know someone who works in a molecular biology lab and is willing to train you and let you work there you could culture your cheek cells, but otherwise it would cost many thousands of dollars to buy a tissue culture hood, incubator, centrifuge, microscope, etc., and set up a lab; and you could not do it in your home.
Mammalian cells like those from your cheek will not grow on agar like bacteria. They have to be cultured in special liquid media with specific growth factors and this has to be done under sterile conditions or bacteria will quickly take over the culture. We occasionally culture cells from mice but normally we use what are called cell lines because they are adapted to culture conditions. We purchase these and keep them sterile by working carefully in the hood.
You can examine cheek cells under the microscope and stain them with different dyes to see various organelles or watch them undergo cell division. You could keep them alive for a couple of hours in saline, but you won't be able to culture them.
Hope this helps.
Sybee
If you know someone who works in a molecular biology lab and is willing to train you and let you work there you could culture your cheek cells, but otherwise it would cost many thousands of dollars to buy a tissue culture hood, incubator, centrifuge, microscope, etc., and set up a lab; and you could not do it in your home.
Mammalian cells like those from your cheek will not grow on agar like bacteria. They have to be cultured in special liquid media with specific growth factors and this has to be done under sterile conditions or bacteria will quickly take over the culture. We occasionally culture cells from mice but normally we use what are called cell lines because they are adapted to culture conditions. We purchase these and keep them sterile by working carefully in the hood.
You can examine cheek cells under the microscope and stain them with different dyes to see various organelles or watch them undergo cell division. You could keep them alive for a couple of hours in saline, but you won't be able to culture them.
Hope this helps.
Sybee
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SciB
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Re: cells
You are welcome!
Please ask again if you need more help.
Sybee
Please ask again if you need more help.
Sybee
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deleted-457439
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Re: cells
Hi,
I live in the the gta and Toronto area. I was wondering if you have any connections to labs around the area.
thanks.
I live in the the gta and Toronto area. I was wondering if you have any connections to labs around the area.
thanks.
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deleted-457439
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Re: cells
also what cell would you recommend using because I am trying to grow it on a leaf to have it used in the future as tissue.
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SciB
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Re: cells
Sorry, I don't know anyone in the Toronto area, but there are certainly some good research labs there. You should do some online searching to find out who is doing what and choose a few professors to contact. Just be sure you are very familiar with their work before you call and that you can explain your project clearly and accurately.
OK. You lost me on your last question. You want to grow cells on a leaf? Can you explain in a little more detail what your hypothesis is? Mammalian cells and plant cells are not very compatible.
Please post again with more information.
Sybee
OK. You lost me on your last question. You want to grow cells on a leaf? Can you explain in a little more detail what your hypothesis is? Mammalian cells and plant cells are not very compatible.
Please post again with more information.
Sybee
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deleted-457439
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- Joined: Thu Nov 30, 2017 4:29 pm
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- Project Question: health sciences
- Project Due Date: march,13,2018
- Project Status: I am conducting my research
Re: cells
Sorry Sybee,
Current bio engineering techniques like 3D printing cant make a vascular network to give the oxygen, nutrients, and needed molecules to grow tissue.
I am planning to decellularize the leaf with sls powder. This will leave a cellulose, I will culture whatever sort of cell is available and leave the leaf in the cells.My hypothesis is that the leaf is going to contract the cells.
Then, I will run colored water and micro beads to check if there can be blood flow through the veins. This will essentially be a tissue. I will first conduct a test to see durability of different leafs.
this might be very unclear please ask more questions so I can clarify.
thanks greatly appreciated,
Emily.
Current bio engineering techniques like 3D printing cant make a vascular network to give the oxygen, nutrients, and needed molecules to grow tissue.
I am planning to decellularize the leaf with sls powder. This will leave a cellulose, I will culture whatever sort of cell is available and leave the leaf in the cells.My hypothesis is that the leaf is going to contract the cells.
Then, I will run colored water and micro beads to check if there can be blood flow through the veins. This will essentially be a tissue. I will first conduct a test to see durability of different leafs.
this might be very unclear please ask more questions so I can clarify.
thanks greatly appreciated,
Emily.