i have a subject

[deleted-666750]

I am doing a study on antioxidants and stress in fruit flies.
For this experiment, I need to stress out my fruit flies (but not kill them), and this will be a major part of my project. I was going to use heat, and put the fruit flies in an incubator at a certain heat for a certain period of time to stress them out.
However...... My teacher just told me I couldn't use this method. What else could I do to stress out my fruit flies (Drosophila Melanogaster)??? A quick response would be much appreciated.
Thank you so much.

[SciB]

I don't understand why you are not allowed to use heat. Did your teacher say WHY? Heat is a common way to induce stress in Drosophila without harming them.

I think cold stress will also work and so will nutritional deprivation, but you will have to look up the correct conditions so that you know that you are inducing stress, but not doing permanent damage.

Sybee