DNA extraction

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deleted-751572
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Joined: Wed Sep 04, 2019 5:37 am
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DNA extraction

Post by deleted-751572 »

Recently I've learned how to extract DNA from vegetables.
When me and my class were in the lab, we were devided into teams.
My team used red sweet pepper/ bell pepper(however you call it).
Every team that used the peppers, got bad results- the DNA came out as red dots instead of white strings.
However, the teams that used onions or cucumbers got perfect white strings(as it should).
I wanted to ask- did it happen for a reason?(are peppers somehow different from other vegetables?) or was there just a problem with the peppers?
deleted-547689
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Joined: Sat Jan 20, 2018 4:51 pm
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Re: DNA extraction

Post by deleted-547689 »

When you say you got "bad results", is this only because your DNA was red? More importantly, was your red DNA thick and goopy? That is a good measure of whether you have DNA or not. If it was simply a red, goopy mixture I would say your extraction was a success. The red pigment from the pepper may have simply been because the pigment was soluble in your extraction buffer. That is not an indication of no success.
deleted-547689
Former Expert
Posts: 31
Joined: Sat Jan 20, 2018 4:51 pm
Occupation: Teacher

Re: DNA extraction

Post by deleted-547689 »

DrJay wrote:When you say you got "bad results", is this only because your DNA was colored? More importantly, was your red DNA thick and goopy? That is a good measure of whether you have DNA or not. If it was simply a colored, goopy mixture I would say your extraction was a success. The red pigment from the pepper may have simply been because the pigment was soluble in your extraction buffer. That is not an indication of no success.
deleted-751572
Posts: 2
Joined: Wed Sep 04, 2019 5:37 am
Occupation: Student

Re: DNA extraction

Post by deleted-751572 »

DrJay wrote:When you say you got "bad results", is this only because your DNA was red? More importantly, was your red DNA thick and goopy? That is a good measure of whether you have DNA or not. If it was simply a red, goopy mixture I would say your extraction was a success. The red pigment from the pepper may have simply been because the pigment was soluble in your extraction buffer. That is not an indication of no success.
Thanks for replying!
I understand about the pigment but there was another difference-
The bell pepper's DNA came out as little dots while the onion's and the cucumber's came out as long strings.
I wanted ask- did it actually happen for a scientific reason or was there just a problem with the experiment?
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