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gel electrophoresis?
Moderators: AmyCowen, kgudger, bfinio, MadelineB, Moderators
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scienceiscoolkinda
- Posts: 2
- Joined: Sat Jan 04, 2014 10:37 pm
- Occupation: student: 8th grade
- Project Question: Gel Electrophoresis.
- Project Due Date: 1/6/13
- Project Status: I am finished with my experiment and analyzing the data
gel electrophoresis?
Ok so I did the building the gel electrophoresis chamber with food dye. I still dont get what bands are. Is it the differrnt shades the food color changes when it runs? What if just stays as one? I'm so confused. ! My ptoject is due in a couple days and if you could answer asap it would help tons. Thank you
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fabulousquantumpeewe
- Posts: 5
- Joined: Thu Dec 26, 2013 4:17 pm
- Occupation: student 8th
- Project Question: Help with Gel Electrophoresis
- Project Due Date: January 6th
- Project Status: I am finished with my experiment and analyzing the data
Re: gel electrophoresis?
lol yeah its the different color bands you get (im doing same experiment) oh by the way what did you do as the control?
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scienceiscoolkinda
- Posts: 2
- Joined: Sat Jan 04, 2014 10:37 pm
- Occupation: student: 8th grade
- Project Question: Gel Electrophoresis.
- Project Due Date: 1/6/13
- Project Status: I am finished with my experiment and analyzing the data
Re: gel electrophoresis?
oh cool! would you mind sharing your results so i can compare mine to yours? idk i have a weird feeling i did something wrong 
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deleted-140482
- Former Expert
- Posts: 186
- Joined: Fri Aug 09, 2013 12:56 pm
- Occupation: Postdoctoral Fellow
- Project Question: Signing up to be an Expert
- Project Due Date: n/a
- Project Status: Not applicable
Re: gel electrophoresis?
Hi,
I assume the project you are talking about doing is this one: https://www.sciencebuddies.org/science- ... ml#summary.
The point of this project is that most of the food colors we see are made by mixing multiple food dyes. Each of these food dyes has a different weight, and we can use gel electrophoresis to separate out these dyes by their weight. So when you run the food dyes from a green M&M (for example), you will see multiple bands of different colors at different places on the gel, and these are the different dyes used to make your green color. If you only get one band on the gel, than that implies that there was only one food dye used to make your food color. You also would have only one band in your controls, which should be a food dye alone, not dyes isolated from a candy.
I hope this helps explain your results. Please feel free to post here again if you have more questions!
JMP
I assume the project you are talking about doing is this one: https://www.sciencebuddies.org/science- ... ml#summary.
The point of this project is that most of the food colors we see are made by mixing multiple food dyes. Each of these food dyes has a different weight, and we can use gel electrophoresis to separate out these dyes by their weight. So when you run the food dyes from a green M&M (for example), you will see multiple bands of different colors at different places on the gel, and these are the different dyes used to make your green color. If you only get one band on the gel, than that implies that there was only one food dye used to make your food color. You also would have only one band in your controls, which should be a food dye alone, not dyes isolated from a candy.
I hope this helps explain your results. Please feel free to post here again if you have more questions!
JMP