Daphnia

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princess77477
Posts: 1
Joined: Thu Jan 16, 2014 5:38 pm
Occupation: Student
Project Question: I am doing the effects of polluntants on Daphnia for my science project. I am having trouble understnding hw to make the dillutions. I am using oil, weed killer and saly. how much water should I add to each mixture if I am using 150 ml of the pollutants
Project Due Date: 1/22
Project Status: I am just starting

Daphnia

Post by princess77477 »

Hello Expert.

I am preparing for my Science Project and I am doing the project the Effects of Pollutants on Daphnia. I am having trouble understanding how to dillute the chemical. If I am using 150 ml of the chemical how much water to add to each mixture. I am using weed killer, antifreeze, oil and salt (150 mg).

Please help.

Amirah :shock:
Adean2001
Former Expert
Posts: 13
Joined: Fri Sep 20, 2013 9:31 am
Occupation: Stay at home mom
Project Question: Registering for ask an expert
Project Due Date: N/a
Project Status: Not applicable

Re: Daphnia

Post by Adean2001 »

Hi Amirah,

It really depends on the concentrations of the chemicals you're using. You have told us that you are adding 150mg of salt (NaCl) to 150mL of water. Based on that information (and using the atomic weight for NaCl, 58.4430g), I can determine that the final molarity of your solution will be 17.1107mM. That means that the rest of your solutions will have to be diluted to this same concentration. I found a great online tool for determining dilutions/molarity http://www.graphpad.com/quickcalcs/Molarityform.cfm . If you need help figuring out how to do the dilutions, you can always repost once you've told us the original concentrations of the solutions you are using. I hope this helps.

Good luck!
Alexis
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