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Can I (1)purchase an enzyme to cut human DNA from human saliva?
And then would I be able to (2)stain it and run it on a homemade electrophoresis (9 volt batteries)?
(after centrifuging the samples)
Do you email back your response????
Thanks!
DNA
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Re: DNA
Hi there,
First of all, it would be helpful to know what your main question is and how you're going to answer it using the experiment you described here.
Yes, you can indeed purchase an enzyme to cut human DNA from saliva. You can find these enzymes on Carolina Biologicals--it seems that they only have three different ones available. Each of these enzymes will cut the DNA at different sequence sites. http://www.carolina.com/biotechnology-e ... tid=235790
First of all, you would need to extract the DNA from the saliva sample and then set up a reaction to digest it with the enzyme. Why do you want to digest the DNA with the enzyme and then run it on a gel? Why do you want to centrifuge the samples before running it on the gel? Do you have the materials to make the gel for running the DNA? Do you have a teacher or mentor who is guiding you as you do these experiments? Are you planning on visualizing the DNA bands on the gel after you run it? If so, this requires some more sophisticated equipment and reagents that are probably only accessible in labs (unless, other experts, you know of homemade gel imaging machines).
Let us know more details so we can provide more helpful advice for your questions.
Best,
Connie
First of all, it would be helpful to know what your main question is and how you're going to answer it using the experiment you described here.
Yes, you can indeed purchase an enzyme to cut human DNA from saliva. You can find these enzymes on Carolina Biologicals--it seems that they only have three different ones available. Each of these enzymes will cut the DNA at different sequence sites. http://www.carolina.com/biotechnology-e ... tid=235790
First of all, you would need to extract the DNA from the saliva sample and then set up a reaction to digest it with the enzyme. Why do you want to digest the DNA with the enzyme and then run it on a gel? Why do you want to centrifuge the samples before running it on the gel? Do you have the materials to make the gel for running the DNA? Do you have a teacher or mentor who is guiding you as you do these experiments? Are you planning on visualizing the DNA bands on the gel after you run it? If so, this requires some more sophisticated equipment and reagents that are probably only accessible in labs (unless, other experts, you know of homemade gel imaging machines).
Let us know more details so we can provide more helpful advice for your questions.
Best,
Connie