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Measuring Apple Slice Brownness
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Measuring Apple Slice Brownness
I am currently preparing for an experiment on the enzymatic browning of apples. What I intend to do is to determine the effect of natural preservatives (i.e lemon) on the browning of apples. However, a problem that I am facing is that I do not know how to quantify my results. Any help?
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deleted-291782
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Re: Measuring Apple Slice Brownness
Hi Nadia,
If you want to be able to quantify your results, you could use an image analysis program such as ImageJ. This is a program from the NIH, and seems like it is a free download:
https://imagej.nih.gov/ij/
Here are some instructions for specifically what you are trying to do:
https://www.unige.ch/medecine/bioimagin ... cation.pdf
What you could do is calculate the intensity of a control apple as a baseline from your pictures. You can also calculate the intensity of your test apples, where this intensity is normalized to your controls to make a bar graph. Let us know if you have further questions about how to do this.
-Pharma
If you want to be able to quantify your results, you could use an image analysis program such as ImageJ. This is a program from the NIH, and seems like it is a free download:
https://imagej.nih.gov/ij/
Here are some instructions for specifically what you are trying to do:
https://www.unige.ch/medecine/bioimagin ... cation.pdf
What you could do is calculate the intensity of a control apple as a baseline from your pictures. You can also calculate the intensity of your test apples, where this intensity is normalized to your controls to make a bar graph. Let us know if you have further questions about how to do this.
-Pharma