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I am trying to silence a C. Elegan gene but I am not sure where to find or make the mutants.. I don't know how because the general instructions online aren't descriptive. The science buddies kit online is for an entire class and not for an individual student, I am not sure where to start but I have done so much research.. I need someones help.
PLEASE respond !
Thank you,
Angie Rahman
HELP RNAi SCI FAIR PROJECT - How to make a mutant to silence a gene
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Re: HELP RNAi SCI FAIR PROJECT - How to make a mutant to silence a gene
Hi Angie. I have molecular biology experience and will try to help you. I did a quick search for RNA interference in C elegans and found several papers describing how to use double-stranded RNA (dsRNA) to silence a gene by blocking translation of complimentary mRNAs. Here's one example: http://labs.bio.unc.edu/Goldstein/Dudle ... nMMB05.pdf
Is that the method you were planning to use for RNAi?
Which gene are you trying to silence? Have you worked with C elegans before? Are you experienced in using equipment and reagents in a research lab setting? Will you be able to do your experiments in a well-equipped lab? I need to know where you will be working so I will know what types of experiment you will be able to perform and what techniques you will be able to use.
Please post again with more information and I will try to answer your questions and get you on the right track.
Sybee
Is that the method you were planning to use for RNAi?
Which gene are you trying to silence? Have you worked with C elegans before? Are you experienced in using equipment and reagents in a research lab setting? Will you be able to do your experiments in a well-equipped lab? I need to know where you will be working so I will know what types of experiment you will be able to perform and what techniques you will be able to use.
Please post again with more information and I will try to answer your questions and get you on the right track.
Sybee