project continuation help

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jmm0837
Posts: 3
Joined: Wed Sep 10, 2008 5:23 pm
Occupation: Student
Project Question: Ultraviolet light and the effects on bactiria
Project Due Date: Feb, 2008
Project Status: I am just starting

project continuation help

Post by jmm0837 »

I'm having a hard time coming up with an idea to expand my research on last years project. I'm in 8th grade and made it to County, but missed out on state competition.
Last year I studied the effects of various methods used to clean (sanitize) soda cans. I used a clean cloth, bleach solution, UV light, alcohol wipe.
Looking for ideas to expand on this!

Thanks!
MelissaB
Moderator
Posts: 1055
Joined: Mon Oct 16, 2006 11:47 am

Re: project continuation help

Post by MelissaB »

Hi,

How did you measure the cleanliness of the soda cans? By swabbing for bacteria? What did you find in your experiment? Answering those questions will help us to help you expand your project. Off the top of my head, though, I would wonder about other surfaces--does the same sanitizing method work best on other items one might want to sanitize, like a kitchen counter? Alternatively, you could look into why the method that worked best worked best, though depending on your results and resources that might be difficult.
jmm0837
Posts: 3
Joined: Wed Sep 10, 2008 5:23 pm
Occupation: Student
Project Question: Ultraviolet light and the effects on bactiria
Project Due Date: Feb, 2008
Project Status: I am just starting

Re: project continuation help

Post by jmm0837 »

I measured the cleanliness of the cans by swabbing each can and recording the amount of bacteria growth in a agar dish. I cleaned the cans using four methods, a clean cloth, alcohol wipe, cloth with bleach solution and placing the cans under a UV light. I expected the UV light to work the best, but my results showed otherwise. Just wiping them with a clean cloth scored higher 3 out of 4 trials.
I'm thinking about possible other surfaces, but also wondered about testing at least three of the four methods on actual bacteria. I've looked for ways to buy petri dishes with a culture growing or grow my own, but not sure.

Thanks!
MelissaB
Moderator
Posts: 1055
Joined: Mon Oct 16, 2006 11:47 am

Re: project continuation help

Post by MelissaB »

Interesting results!

The idea of using actual bacteria is a really interesting one, but you would need to be careful in how you did it. Even 'harmless' bacteria can be very dangerous in large quantities, so you would not want to grow large colonies of bacteria on a plate and then 'clean' them--you could make yourself or someone else very sick. What you could do is take some lunchmeat (NOT raw meat, especially chicken) and place it on, say, a clean breadboard or a countertop. Then clean the breadboard/countertop with one of your four methods, then swab the item and culture the bacteria. That way you could control the amount of bacteria on the item, but you would not be exposing yourself to dangerous amounts of bacteria while you cleaned.

Hope this helps!
deleted-71297
Former Expert
Posts: 8
Joined: Thu Nov 15, 2007 6:32 pm

Re: project continuation help

Post by deleted-71297 »

Hi, I agree, go with whatever bacteria are already there.

Two ideas on how to improve. First, why cans? What makes them interesting? Perhaps you can find another scenario that is more interesting or applied (cutting boards?).

Second, I would be interested to see the differnece between products that claim to be 'antibacterial' and those that don't. I have heard that normal soap is just as good, but haven't tried the test mysef.

Roland
deleted-71615
Former Expert
Posts: 62
Joined: Tue Sep 18, 2007 7:11 pm

Re: project continuation help

Post by deleted-71615 »

One way to expand on your project might be to try different disinfectants (bleach, alcohol, iodine and other commercial disinfectants) at different concentration, to see at what concentration the disinfectant work best. You can do a graph comparing the concentration of the disinfectant and the colonies count.
Michael
deleted-32101
Posts: 20
Joined: Tue Sep 23, 2008 12:27 pm
Occupation: Student
Project Question: what is the animal that contains the most bacteria that a human owns?
Project Due Date: November 19, 2008
Project Status: I am just starting

Re: project continuation help

Post by deleted-32101 »

jmm0837 wrote:I measured the cleanliness of the cans by swabbing each can and recording the amount of bacteria growth in a agar dish. I cleaned the cans using four methods, a clean cloth, alcohol wipe, cloth with bleach solution and placing the cans under a UV light. I expected the UV light to work the best, but my results showed otherwise. Just wiping them with a clean cloth scored higher 3 out of 4 trials.
I'm thinking about possible other surfaces, but also wondered about testing at least three of the four methods on actual bacteria. I've looked for ways to buy petri dishes with a culture growing or grow my own, but not sure.

Thanks!
hi im also having trouble finding cheap petri dishes for my project lol!

im still loooking! if you found a site that has em could you tell me plz cuz my project is due on the 3rd.. any help would be awesome thanks.. :mrgreen:
"my name is bob and you cannot stop me" -- moi
jmm0837
Posts: 3
Joined: Wed Sep 10, 2008 5:23 pm
Occupation: Student
Project Question: Ultraviolet light and the effects on bactiria
Project Due Date: Feb, 2008
Project Status: I am just starting

Re: project continuation help

Post by jmm0837 »

stanthescienceman;
you can get petri dishes from carolina.com they hav 20 plates for $25.95. :D
deleted-32101
Posts: 20
Joined: Tue Sep 23, 2008 12:27 pm
Occupation: Student
Project Question: what is the animal that contains the most bacteria that a human owns?
Project Due Date: November 19, 2008
Project Status: I am just starting

Re: project continuation help

Post by deleted-32101 »

hey thanks for the website... i really needed them for my project..i have a class in school that does nothing but research and science fair for the first semester so that's great!!! can i add u as a friend? 8)

stanthescienceman
"my name is bob and you cannot stop me" -- moi
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