Stopping bacteria growth on agar plates

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JessicaH001
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Stopping bacteria growth on agar plates

Post by JessicaH001 »

Hi, I am doing a science project involving the use of agar plates and bacteria. I have zones of inhibition and wish to bring in my agar plates on presentation day. But I don't want the bacteria to keep on growing, because I am afraid that that will change some of my results that I have already recorded. What can I do to make sure that the bacteria (e. coli) does not grow any more, or grows very slowly?

I was thinking of storing it in a refrigerator at 4 degrees Celsius, but im not 100% sure...
deleted-71827
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Re: Stopping bacteria growth on agar plates

Post by deleted-71827 »

Hi,
There are actually a variety of bacteriostatic agents that you might try and use. Check out this link for a list of such agents!

http://en.wikipedia.org/wiki/Bacteriostatic

Refrigeration will also slow down the rate of bacteria growth, although this may not always work. Best of luck!
"There is a single light of science, and to brighten it anywhere is to brighten it everywhere." -Isaac Asimov
deleted-71615
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Re: Stopping bacteria growth on agar plates

Post by deleted-71615 »

Instead of bringing the actual agar plates, you might consider taking pictures of the plates along with a ruler so others can see the size of zones of inhibition. Keep in mind that the agar plate only have so much nutrient and eventually the bacteria will start dying.

Michael
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