Help needed for a Programmable Probiotic using E. coli Nissle 1917 as a cancer-targeting vector

[Rosa1234]

Hi! My name is Alia and I'm a 12-year-old Grade 5 student from Dhaka, Bangladesh. I've been developing a theoretical concept for a Programmable Probiotic using E. coli Nissle 1917 as a cancer-targeting vector. I call her Karen 1917 (Singular: A Karen 1917, Plural: Karens 1917). I have a question for you. How do I stop the liver and spleen from filtering out 90% of my bacteria before they reach the tumor? I am worried about Hepatic Clearance and Kupffer cell phagocytosis. Should I use Capsular Polysaccharide (CAP) engineering to give her invisibility , or will that add too much metabolic burden and break my cage? This is my current research that is a bit of a failure right now because the liver keeps killing my Karens (my poor Karens, what did they ever do) , but I really want you to check it out and answer my question while keeping it in mind:


(is this research on the right track?)

My Karens are currently failing and it's honestly a vibe kill so could you please help me if it's not too much... Thank you so much for your time!

[Administrator note: link removed.]

[Rosa1234]

Hi! I noticed my research link was removed. I'm sorry! I just wanted to show the experts my data so they could see what I'm working on. Is there a way I can share my research work without any problems? Also, I got an email that said someone replied to me but I can't see it! Please help!

[amyCC]

Hi - I apologize for the confusion about the notification you received. A spam post was made in reply to your post, and it was removed.

I am not sure that we will have an Expert currently helping in the forums who can help with the level of research you are conducting. Are you working in a lab with a mentor?

Amy
Science Buddies

[Rosa1234]

Hi Amy! Thank you for the reply. No, I don't have anyone helping me (except google and a lot of reading materials) let alone a lab or mentor (yet...maybe I'll get one someday who knows) lol. I'm just someone who thinks too much and comes up with weird ideas like this one. Since there might not be an expert here for this level, do you know anyone I could talk to about Hepatic Clearance? I'm really stuck on how to protect my Karens from the liver! Thanks again!