Microbiology Techniques & Troubleshooting
The following Microbiology resources were developed by the Department of Biology at the University of Missouri in St. Louis and are reproduced, without modification, here with their permission. The originals can be found at: http://www.umsl.edu/~microbes/techniques.html
| Microorganisms Safety Guide | A Science Buddies resource |
| Storing Bacterial Strains | How to store a bacterial strain for later use |
| Culture Problems | Descriptions of some common problems and solutions to those problems |
| Sterile Technique | Methods for sterile transfer of cultures in broth or on plates |
| Optimum Temperatures | Optimum temperatures for growth of common lab strains |
| Sterilizing | Methods for sterilizing media and glassware in the classroom |
| Streaking plates | Method for streaking plates to obtain isolated colonies |
| Nutrient Agar | Instructions for preparing nutrient broth, plates, and slants |
| Tips for Pouring and Storing Agar Plates | Tips for pouring and storing plates |
| Incubator | A low-cost incubator for the classroom |
| Homemade Media | An inexpensive alternative to commercial media |
| Testing Autoclaves | Commercial spore solutions test sterilizers |
| Tyndallization | An old-fashioned method for sterilizing broth |
| Simple Staining of Microbes | Instructions for staining bacteria |
Source
University of Missouri, St. Louis Department of Biology. (n.d.). Techniques and Troubleshooting. Science in the Real World: Microbes in Action Program. Retrieved June 30, 2010 from http://www.umsl.edu/~microbes/techniques.html
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