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Microbiology Techniques & Troubleshooting

The following Microbiology resources were developed by the Department of Biology at the University of Missouri in St. Louis and are reproduced, without modification, here with their permission. The originals can be found at: http://www.umsl.edu/~microbes/techniques.html

Microorganisms Safety Guide A Science Buddies resource
Storing Bacterial Strains How to store a bacterial strain for later use
Culture Problems Descriptions of some common problems and solutions to those problems
Sterile Technique Methods for sterile transfer of cultures in broth or on plates
Optimum Temperatures Optimum temperatures for growth of common lab strains
Sterilizing Methods for sterilizing media and glassware in the classroom
Streaking plates Method for streaking plates to obtain isolated colonies
Nutrient Agar Instructions for preparing nutrient broth, plates, and slants
Tips for Pouring and Storing Agar Plates Tips for pouring and storing plates
Incubator A low-cost incubator for the classroom
Homemade Media An inexpensive alternative to commercial media
Testing Autoclaves Commercial spore solutions test sterilizers
Tyndallization An old-fashioned method for sterilizing broth
Simple Staining of Microbes Instructions for staining bacteria


University of Missouri, St. Louis Department of Biology. (n.d.). Techniques and Troubleshooting. Science in the Real World: Microbes in Action Program. Retrieved June 30, 2010 from http://www.umsl.edu/~microbes/techniques.html

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