A basic understanding of molecular biology and access to a laboratory where bacteria can be grown are required for this project.
The kit required for this project can only be purchased through a school or educational business.
High ($100 - $150)
Adult supervision is required in the laboratory facility.
Sandra Slutz, PhD, Science Buddies
For this science project you will need to develop your own experimental procedure. Use the information in the summary tab as a starting place. If you would like to discuss your ideas or need help troubleshooting, use the Ask An Expert forum. Our Experts won't do the work for you, but they will make suggestions and offer guidance if you come to them with specific questions.
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Can bacteria be altered to produce life-saving insulin for diabetics? Or change color to indicate the presence of a harmful toxin? Yes, it can! Using biotechnology, scientists work daily on problems like these. It starts with selecting a gene you want bacteria to produce and creating a sequence of DNA that has that gene and a promoter that will help express it at the right time and at the right levels. The next hurdle is actually getting this engineered DNA into the bacteria and functional. One way of doing that is a method called bacterial transformation. In bacterial transformation, bacteria are treated in a manner which induces some of them to take up a plasmid of DNA and express it. It doesn't work for every bacterial cell though.
Just how is bacterial transformation done? How efficient is it? Does the efficiency change if you use different strains of bacteria? If the bacteria do take up the engineered DNA, is the level of expression always the same? You explore these questions as well as many others about bacterial transformation and the expression of engineered DNA using the
BioBuilder What a Colorful World Kit
from our partners at Carolina Biological.
The kit contains materials to do several transformations of two different strains of E. coli. The plasmids included have a pigment inducing gene under the regulation of different promoters. With the kit and access to a laboratory with bacteria growing equipment like incubators and shakers, you'll be ready to explore some interesting and real-world biotechnology.
Ask an Expert
Do you have specific questions about your science project? Our team of volunteer scientists can help. Our Experts won't do the work for you, but they will make suggestions, offer guidance, and help you troubleshoot.
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Microorganisms (bacteria, viruses, algae, and fungi) are the most common life-forms on Earth. They help us digest nutrients; make foods like yogurt, bread, and olives; and create antibiotics. Some microbes also cause diseases. Microbiologists study the growth, structure, development, and general characteristics of microorganisms to promote health, industry, and a basic understanding of cellular functions.
What do the sequencing of the human genome, the annual production of millions of units of life-saving vaccines, and the creation of new drought-tolerant rice varieties have in common? They were all accomplished through the hard work of biological technicians. Scientists may come up with the overarching plans, but the day-to-day labor behind biotechnology advances is often the work of skilled biological technicians.
A nice cool yogurt is the perfect snack. It comes in a variety of delicious flavors like peach, chocolate, and cherry and contains calcium, vitamins, and minerals that are good for you. Yogurt also contains live cultures that your body needs to maintain good health. How did all of those good things get into your yogurt? The answer is that a biochemical engineer helped to develop a recipe to make that yogurt a perfect snack for you. So many of the products that we use every day, from medicine…
General citation information is provided here. Be sure to check the formatting, including capitalization, for the method you are using and update your citation, as needed.
"Transforming Bacteria to Make Colored Pigments." Science Buddies,
20 Nov. 2020,
Accessed 20 Mar. 2023.
(2020, November 20).
Transforming Bacteria to Make Colored Pigments.