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Summary

Areas of Science
Difficulty
 
Time Required
Average (6-10 days)
Prerequisites
A basic understanding of molecular biology and access to a laboratory where bacteria can be grown are required for this project.
Material Availability
The kit required for this project can only be purchased through a school or educational business.
Cost
High ($100 - $150)
Safety
Adult supervision is required in the laboratory facility. Use sterile technique. Read the Microorganisms Safety Guide before starting any experiments.
Credits
*Note: For this science project you will need to develop your own experimental procedure. Use the information in the summary tab as a starting place. If you would like to discuss your ideas or need help troubleshooting, use the Ask An Expert forum. Our Experts won't do the work for you, but they will make suggestions and offer guidance if you come to them with specific questions.

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Abstract

What is expression cloning and how is it used in the biotechnology industry? How are plasmids constructed for use in a bacterial expression system? You can investigate these questions and more using bacterial expression kits meant for high school classes. You will need a laboratory space equipped to grow bacteria and carry out simple molecular biology. A kit with plasmids and bacterial cells to transform are also needed. Here are two possibilities:



Bacteria in a petri dish glows green due to the green florescent protein
Figure 1. Using biotechnology techniques like expression cloning, bacteria can be engineered to express many different proteins like the green florescent protein, GFP, which make these bacteria glow green. (Photo courtesy of Bio-Rad Laboratories, Inc.)
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General citation information is provided here. Be sure to check the formatting, including capitalization, for the method you are using and update your citation, as needed.

MLA Style

Science Buddies Staff. "Expression Cloning." Science Buddies, 20 Nov. 2020, https://www.sciencebuddies.org/science-fair-projects/project-ideas/BioChem_p024/biotechnology-techniques/expression-cloning. Accessed 22 May 2022.

APA Style

Science Buddies Staff. (2020, November 20). Expression Cloning. Retrieved from https://www.sciencebuddies.org/science-fair-projects/project-ideas/BioChem_p024/biotechnology-techniques/expression-cloning


Last edit date: 2020-11-20
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