Expression Cloning *
|Time Required||Average (6-10 days)|
|Prerequisites||Some laboratory experience is required: knowledge of sterile technique, working with bacterial cultures, and using automatic pipettes are all helpful.|
|Material Availability||This science project requires the purchase of Bio-Rad's pGLO Bacterial Transformation Kit. Details about how to order products from Bio-Rad can be found here.|
|Cost||Very High (over $150)|
|Safety||Adult supervision is required in the laboratory facility. Use sterile technique. Read the Microorganisms Safety Guide before starting any experiments. SRC approval may be necessary.|
AbstractWhat is cloning and how is it used in the biotechnology industry? How are plasmids constructed for use in a bacterial expression system? You can investigate bacterial expression with the pGLO Bacterial Transformation Kit from the Bio-Rad Biotechnology Explorer Program to make bacteria express green fluorescent protein (GFP), as shown in Figure 1, below (see the Science Buddies project, Bacterial Transformation Efficiency). With variations to this kit you can test the conditions for pGLO expression under different conditions. You can also combine with other kits to map the pGLO plasmid and purify the expressed pGLO protein using column chromatography. (Bio-Rad, 2006)
Figure 1. You can investigate bacteria expressing GFP using the pGLO Bacterial Transformation Kit. (Photo courtesy of Bio-Rad Laboratories, Inc.)
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Last edit date: 2017-07-28
BibliographyBio-Rad, 2006. "Biotechnology Explorer Program," Bio-Rad Laboratories [accessed January 5, 2009] http://explorer.bio-rad.com
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