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Waste Not, Want Not: Use a Microbial Fuel Cell to Create Electricity from Waste

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"Gross! What is that in the toilet?" But maybe it's not just gross. Did you know there are bacteria that digest organic waste and create electrons? What if there was a way to collect those electrons to power a circuit? In this science fair project, you will make a microbial fuel cell to collect the electrons that the bacteria—anaerobic bacteria—create...only, you'll be using mud, which is much safer to handle than wastewater. If you would like to learn how to reuse and recycle an unlikely substance, this is the science fair project for you!


Areas of Science
Time Required
Very Long (1+ months)
Previous experience using a multimeter and being familiar with the physics of electricity is helpful, but not required.
Material Availability
A kit is available from our partner Home Science Tools. See the Materials section for details. You also need to have access to a lower-order stream of water or a creek. See the Introduction for an explanation of this type of stream. Try to avoid streams in which the bed is rocky. The benthic mud sample should be from an area that has a thick, rich mud bed.
High ($100 - $150)
Adult supervision is recommended. Be careful when using a hot stove and a drill. Remember to always wear safety goggles when drilling. Exercise caution when working near a stream or creek. Water currents can be stronger than they look.

Michelle Maranowski, PhD, Science Buddies

This project idea is based on the following 2008 California State Science fair project, a winner of the Science Buddies Clever Scientist Award: Bennet, I. (2008). Generating Electricity from Wastewater Using a Microbial Fuel Cell. Retrieved August 23, 2008.

The design in this science fair project is based on Abbie Groff's design in her science fair project, titled "Identification of Benthic Microbes Utilizing Bioremediation and Microbial Fuel Cells."

The author would like to thank Sandra Slutz, PhD, Science Buddies, for very helpful discussions.

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To learn about an alternative method for creating electricity, the microbial fuel cell. The goal is to build a microbial fuel cell using a benthic mud sample from a stream and determine if this device can harvest the electrons that the anaerobic bacteria (present in the mud sample) create.


In order to reduce pollution, we need to develop alternative and renewable energy sources. When people think of alternative and renewable energy sources, they usually think of harvesting energy from the sun (solar energy), earth (geothermal energy), water (hydropower), or wind. But by using a microbial fuel cell (MFC), electricity can be extracted from wastewater! The microbial fuel cell converts organic material to electricity using bacteria, leaving behind clean drinking water in the process. This is an exciting prospect for people around the world who lack adequate sanitation and the means to afford it. In addition, water treatment plants require a lot of power to treat water. Each year, almost 25 billion dollars are spent in treating wastewater. A lot of money and resources could be saved if the wastewater could be used as a fuel!

The microbial fuel cell is a bio-electrochemical system in which bacteria are used to convert organic material into electricity. There exist many different microbial fuel cell designs such as one-chambered or two-chambered MFCs. The traditional H-shaped two-chambered microbial fuel cell is made of several components: the two electrodes (the anode and the cathode), a proton-exchange membrane (PEM) and an external circuit. The anode chamber holds the bacteria and organic material in an anaerobic (without oxygen) environment. Here, the anaerobic bacteria consume the organic waste material while extracting electrons from their food source and oxidizing it to carbon dioxide. As part of their digestive process, the bacteria create protons (H+) and electrons (e-). This process is also known as oxidation. The electrons are pulled out of the solution onto the anode and are conducted through an external circuit into the cathode. The cathode chamber holds a conductive saltwater solution. The protons generated by the bacteria travel through the proton-exchange membrane (PEM) or a salt bridge to meet with the electrons at the cathode. The PEM or salt bridge separates the anode and cathode chambers, but at the same time allows protons to move from one electrode chamber into the other. At the cathode, the protons and electrons combine with oxygen to create water. All the processes happening in a microbial fuel cell are summarized in Figure 1. To read more about the basics of electricity, see the Science Buddies Electricity, Magnetism, & Electromagnetism Tutorial.

Diagram of a microbial fuel cell

Diagram of a microbial fuel cell that uses a proton-exchange membrane to allow hydrogen ions to pass between the anode and cathode side of the cell. Incoming waste water brings bacteria which attach to an anode and produce electrons and hydrogen ions. The electrons travel up the anode to an external circuit and flow back down into the cathode. Hydrogen ions pass through a membrane that separates the anode from cathode and react with oxygen on the cathode side of the cell which forms water that exits from the cathode side of the fuel cell.

Figure 1. This diagram shows how a microbial fuel cell (MFC) functions.

There are two kinds of microbial fuel cells: mediator and mediator-less. In a mediator microbial fuel cell, the bacteria are electrochemically inactive. The bacteria digest the organic material and create electrons. However, the bacteria have no mechanism to rid themselves of the electrons. This is where the mediator helps. The mediator is an inorganic substance, such as thionine, humic acid, or methylene blue, which crosses the membrane of the bacteria and frees the electrons. The mediator then carries the electrons away from the bacteria and deposits them on the electrode. One disadvantage to the mediator microbial fuel cell is that many of the mediators are toxic.

In a mediator-less microbial fuel cell, the bacteria are electrochemically active. The electrochemically active bacteria, also called electrogenic bacteria carry the electrons they create through digestion of organic material to the electrode. There are several hypotheses how this electron transfer works including the generation of nanowires or direct electron transfer through special outer membrane proteins. However, the detailed mechanisms of how electrogenic bacteria transfer electrons are still not completely understood.

In this science fair project you will build a mediator-less microbial fuel cells. Since working with wastewater samples can be challenging, you will use a mud sample. The mud sample will be from a local lower order stream or creek. A lower order stream is one that is formed by the joining of other streams. So a first-order stream is one that does not have any other streams feeding into it. When two first-order streams merge, they create a second-order stream, etc. The mud sample will be taken from the floor of a second-order (or lower) stream. This area of the stream is called the benthic zone. Both, the wastewater sample and the benthic zone mud sample contain anaerobic bacteria that are electrochemically active. In fact, wastewater, benthic mud and even simple soil is packed with bacteria that generate electricity when placed in a microbial fuel cell (MFC). Because such bacteria-laden soil and mud is found almost everywhere on Earth, microbial fuel cells can make clean, renewable electricity nearly anyplace around the globe.

Do you think that electricity can be harvested from mud? In the case of this MFC, seeing is believing. Have fun experimenting, and remember that you are working in an area that is contributing to the well-being of our planet Earth.

Terms and Concepts



The following website has a lot of great information about the microbiology and electrochemistry of microbial fuel cells.

This website details the work being conducted at Pennsylvania State University. It also shows several pictures of homemade microbial fuel cells, including one built by Ian Bennet, as well as instructions on how to build one.

NASA is interested in reusing human waste. Read this website to learn how!

  • Miller, K. (2004, May 18). Waste Not. Retrieved August 15, 2008.

Watch this video in which Prof. Bruce Logan explains the technology and applications of a microbial fuel cell:

Materials and Equipment Buy Kit

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Note: In this science project you will use the materials listed below to build your own microbial fuel cell. If you want to use a pre-made microbial fuel cell to test your benthic mud for electricity generation, or if you want to compare two different microbial fuel cell designs (one-chambered versus two-chambered), you can also purchase MudWatt Classic Kit (2) from our partner Home Science Tools.

To build your own microbial fuel cell you will need:

Building the Anode and Cathode Containers

Making the Electrodes

Making the Salt Bridge

Obtaining the Benthic Mud Samples

Assembling the Fuel Cell

Testing the Fuel Cell

Disclaimer: Science Buddies participates in affiliate programs with Home Science Tools, Amazon.com, Carolina Biological, and Jameco Electronics. Proceeds from the affiliate programs help support Science Buddies, a 501(c)(3) public charity, and keep our resources free for everyone. Our top priority is student learning. If you have any comments (positive or negative) related to purchases you've made for science projects from recommendations on our site, please let us know. Write to us at scibuddy@sciencebuddies.org.

Experimental Procedure

Microbial Fuel Cells in Science Fairs

Working with microbial fuel cells involves growing soil bacteria. Because of this, many science fairs, including those associated with the International Science and Engineering Fair (ISEF) have requirements which need to be met before you start your project. We recommend you:

  • Check with your teacher or science fair coordinator about any requirements
  • Read the Science Buddies Microorganisms Safety Guide to learn how to safely handle bacteria

The goal of this science fair project is to determine if an MFC using a benthic mud sample can create electrons or electricity. You can either do this by building your own two-chambered microbial fuel cells as described in the procedure below or you can use one-chambered microbial fuel cells that are available for purchase from the Science Buddies Store. If you are using the microbial fuel cell kit, you can follow the setup instructions found in the procedure of this science project: Powered by Pee: Using Urine in a Microbial Fuel Cell. The following experimental procedure states to perform actions several times, which means that you will build three identical microbial fuel cells in total. Keep this in mind as you go through the procedure, and also keep in mind that you will be asked to perform three parallel trials. All strong science fair projects are replicated at least three times.

The procedure is broken into six sections: Building the Anode and Cathode Chambers, Making the Electrodes, Making the Salt Bridges, Obtaining the Benthic Mud Sample, Assembling the Microbial Fuel Cells, and Testing the Microbial Fuel Cells.

Note: Once you collect the mud samples, you will need to use them within 24 hours. Make sure you have everything you need to assemble the microbial fuel cells and start your experiment before collecting the samples. Since you don't want to take too many trips to your local stream, get enough of a benthic sample to use for three parallel trials.

Building the Anode and Cathode Chambers

  1. Unscrew the two ends of the compression fitting and discard the rubber fitting. Using the sandpaper, roughen the endcaps of the compression fitting as shown in Figure 2.
Plastic endcap rubbed against sandpaper
Figure 2. Roughening one of the endcaps.
  1. Take the sandpaper and roughen two opposing sides of two of the plastic containers. Just roughen two 1-inch by 1-inch patches across from each other. Making a rough patch will help join all of the parts together.
  2. Using the permanent marker, make a mark in the center of the roughened side of one of the plastic containers. Use the ruler to measure the location of the mark and then make a mark at exactly the same location on one of the roughened sides of a second plastic container. Make sure that the marks are exactly opposite of and facing each other.
  3. Using the ruler, measure the outer diameter of the aquarium air pump tubing. Record this number in your lab notebook.
  4. Put on the safety goggles. Carefully drill a hole 2 millimeters (mm) in diameter on top of two of the plastic container lids. Brush off any plastic debris from the lids. In one of those lids, drill another hole the same diameter as the plastic tubing of the aquarium air pump next to the first hole.
  5. Using the 3/4-inch spade drill bit, drill a hole on the permanent marker marks on the sides of both plastic containers. Note: Drill slowly or the acrylic might crack. Brush off any plastic debris. You should now have two plastic containers that each have a hole in one side as shown in Figure 3.
A hole is drilled into the side of a plastic box
Figure 3. Plastic container with a hole drilled into the side.
  1. Read the safety recommendations on the acrylic cement. Squeeze acrylic cement around one of the 3/4-inch spade drill bit holes. The acrylic cement is watery so be careful not to get it in the hole or on your fingers. Now squeeze acrylic cement around the flat part of one compression fitting endcap. Center the endcap over the hole on one of the plastic containers that you just squeezed cement around. Fit the two pieces together. Hold the two together for 30 seconds. Squeeze some additional cement around the outside of the endcap where it joins with the container. This is to ensure that you minimize liquid leaks. Let the assembly dry for 10 hours.
  2. After 10 hours, screw in the tube as tightly as possible. Hold the endcap firmly and just screw in the tube.
  3. Now screw the second roughened endcap into the tube. Make sure to tighten the endcap firmly.
  4. Lay the second container on its side with the 3/4-inch spade drill bit hole face up. Squeeze acrylic cement around the hole, making sure not to get any in the hole or on your fingers. Squeeze some acrylic cement on the second endcap on the assembly. Position the endcap and assembly over the 3/4-inch spade drill bit hole in the second container. Make sure that it is centered. Use the ruler to make sure that the containers are straight and level. Make adjustments as necessary. You need to make sure that the containers can sit flat on the table when all the parts are dry. Hold the entire assembly together for 30 seconds. Squeeze some acrylic cement around the outside of the endcap where it joins with the second container. Let the entire assembly (the two containers with connecting compression fitting), dry for 10 hours. See Figure 4 for a completed assembly.
A plastic tube connects two plastic containers

The plastic tube is attached over a hole drilled into the side of each plastic container. Acrylic cement is used to secure the plastic tube so that no liquids leak out.

Figure 4. Aligning and cementing the second endcap and container.
  1. After 10 hours have elapsed, check to see if the two joints are watertight. Fill the containers with water past the holes/joints. Wait for 5 minutes. If there is no water leaking out, then proceed to the next section. If there is excess water coming out of a joint, empty the containers and dry them off completely with paper towels. Carefully squeeze acrylic cement around the endcap joint that leaked. Squeeze out enough cement that you make a seal, but not so much that it becomes messy or that you seal the tube. Wait for 10 hours and retest the watertightness. Try again if this doesn't work. If it still doesn't work, remake the assembly with fresh parts.
  2. Once the parts are watertight, carefully unscrew the tube from the endcaps. Set the tube aside.
  3. Repeat steps 1-12 two more times. You should end this section with three sets of an anode and cathode chamber, like the one shown in Figure 5.
A plastic tube connects two plastic containers
Figure 5. This is one set of a connected anode and cathode chamber.

Making the Electrodes

  1. With the scissors, cut the carbon cloth into four equal squares. Each square should be 5 cm x 5 cm.
  2. Take each of the four pieces of copper wire and with the wire strippers, strip off 6 inches of the insulator on one end of each piece. Strip off 1 centimeter (cm) from the other end of each wire.
  3. Prepare the nickel epoxy according to its directions.
  4. Epoxy the 6 inches of bare copper wire to the carbon cloth along the edges of the square. Repeat with the other three carbon cloths. Let the epoxy harden for 10 hours.
Drawing of a wire epoxied to the inner edge of a square carbon cloth electrode
Figure 6. This is a visual of how the electrode should look.
  1. Once the epoxy has hardened, test the connection between the carbon cloth square and the copper wire with the digital multimeter. If you need help using a multimeter, check out the Science Buddies reference How to Use a Multimeter. Turn the digital multimeter to the resistor/resistance mode. Place one lead on the carbon cloth and the other lead on the free bare end of the copper wire. There should be no or very low resistance (1–3 ohms). If there is a large resistance, then remake the electrode.
  2. Repeat steps 1–5 one more time. You need to make enough electrodes to make three anode-cathode pairs. In other words, you need to make six electrodes. At the end of this section, you will have eight electrodes. Set two of the electrodes aside and keep them as spares.

Making the Salt Bridges

  1. Place some plastic wrap along the bottom of a petri dish so that the ends of the plastic wrap are overlapping the edges of the petri dish. Set the covered dish aside.
  2. Cover one end of the tube section (that you removed in step 12 of the Building the Anode and Cathode Chambers section) from the compression fitting securely with aluminum foil. Repeat with the tubes from all of the compression fittings. Place all tubes, open end up, vertically on the petri dish and then set it aside.
  3. Measure 300 milliliters (mL) of water and pour it into the pot.
  4. Using the scale, measure out 30 g of agar. Set the measured agar aside. Now measure out 6 g of salt.
  5. Place the pot of water on the stove and bring it to a boil. When the water is boiling, add the agar and stir it with the glass rod until it is dissolved.
  6. Once the agar is dissolved, take the pot off of the heat and add 6 g of salt. Stir with a spoon until the salt is dissolved.
  7. While the solution is still warm, carefully pour the solution into the tubes in the petri dish. If the tubes leak, tighten the foil and refill them. Once the tubes are filled and stable (i.e. haven't fallen over, leaked liquid, etc.) for 10 minutes, carefully move the petri dish to the refrigerator. Let the tubes sit in the refrigerator overnight. These tubes are the salt bridges.
  8. The next day, come back and place the salt bridges into a 1-qt. plastic baggie and seal it. This prevents the salt bridges from drying out. They should be a firm, jelly-like substance but not completely solid. Take the bridges out when you are ready to use them.

Obtaining the Benthic Mud Sample

  1. Go to the location of your stream where you have found the richest riverbed. The sample you get should not be full of rocks or twigs, just rich mud.
  2. Tie the nylon rope securely around the middle of the PVC tube.
  3. Throw the tube into the stream or creek. Make sure that you keep a good hold onto the end of the rope! Try to scoop as much of the stream bed as you can.
  4. When you think that you have gotten a large enough sample, drag the tube back to shore. Gently tap the pipe with the hammer and transfer the sample into the bucket. Cover the bucket with plastic wrap and set it aside. Make sure to get enough of the benthic sample to fill the anode chamber and remember to use the sample within 24 hours.
  5. Collect some of the stream water in the cleaned 1-gallon jug. Be careful when retrieving this sample. Always exercise caution when you are near a stream or a creek, as the water current can be stronger than it looks.

Assembling the Microbial Fuel Cells

  1. Retrieve the six containers built in section 1 and the salt bridges made in section 3. Remove the aluminum foil from the salt bridges. Connect a pair of containers with a salt bridge. Repeat two more times. You now have set up three microbial fuel cells containing an anode and cathode chamber each connected with a salt bridge.
  2. Make a conductive salt solution using the water sample from the 1-gallon jug. Measure out 12 cups of stream water into the large bowl. Add 6 Tbsp. of salt to the bowl and stir with a spoon until the salt has been dissolved. Fill the cathode chambers of the three microbial fuel cells with the salt solution.
  3. Take an electrode (this will be your cathode) and thread it through the smaller hole of one of the lids with two holes. Place the lid with the two holes and the connected cathode back onto the cathode chamber. Make sure the electrode is submerged. Repeat this step with another electrode and the other lids with the two holes. Seal each cathode chamber with a lid.
  4. Connect the tubing to the outlet of the aquarium pump. Push the tubing into the cathode chambers through the larger hole in the lid. Be sure to submerge the end of the tubing.
  5. Now, wearing gloves and safety goggles, fill half of the anode chamber of a microbial fuel cell with the benthic mud sample. Make sure that there are no bubbles in the mud. Push the mud sample down or gently tap to remove any bubbles. Take one of the electrodes (this will be your anode) and bury it in the mud. Then place more of the benthic mud into the anode chamber, covering the anode. Push the free end of the electrode copper wire into the 2-mm holes in the container lids. Replace the lid onto the container to make sure that the electrode is hanging freely without hitting any of the walls or the bottom. You can use a little electrical tape on the outside top to hold the electrode in place. Repeat filling the anode chamber with your benthic mud sample and inserting the anode with the other two microbial fuel cells by repeating step 5 for the two additional trials. The microbial fuel cells are now complete and you should have three MFCs in total.

Testing the Microbial Fuel Cells

To evaluate the overall performance of your MFC, you can determine its power output. This is done by measuring the voltage across a fixed resistor that you attach to the MFC and from that, you can calculate power using a derivation of Ohm's Law as shown in Equation 1.

Equation 1:

  • P is the power in watts (W),
  • V is the voltage (V), and
  • R is the resistance in ohms (Ω).
  1. First, turn on the aquarium pump. This aerates the solution in the cathode chamber and creates more oxygen. Leave the pump on for the duration of your experiment.
  2. Then connect the resistor and make the external circuit for the electrons to move through. Take one of the alligator clip cables and clip one end to the bare end of the electrode coming from the anode. Clip the other end of the cable to one end of the 220Ω resistor. Clip the end of the second alligator clip cable to the free end of the resistor. Clip the other end of the cable to the bare end of the electrode coming from the cathode.
  3. Now test the voltage between the anode and the cathode with the digital multimeter. Test the first microbial fuel cell. Turn on the multimeter and put it in "voltage" mode. Measure the voltage reading across the resistor. Is there a voltage reading? You might have to adjust the sensitivity of your voltage setting on your multimeter (mV or μV) depending on your voltage readings. Note down your data in your lab notebook in a data table.
  4. Take voltage readings twice a day at the same times, every day for 30 days.
  5. Repeat steps 1–3 of this section for the other two microbial fuel cells. Always record your data in your lab notebook.

Analyzing Your Data

  1. From your voltage readings each day calculate the power output for each microbial fuel cell using Equation 1.
  2. Plot your results on a scatter plot showing the time on the x-axis and the calculated power output on the y-axis. Make three plots; one for each microbial fuel cell.
  3. Did the microbial fuel cells produce electricity? If yes, did the MFCs start producing electricity right away? Did the electricity production ever peak? How did the electricity production vary over one day?
icon scientific method

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Global Connections

The United Nations Sustainable Development Goals (UNSDGs) are a blueprint to achieve a better and more sustainable future for all.
This project explores topics key to Affordable and Clean Energy: Ensure access to affordable, reliable, sustainable and modern energy.


  • What is the maximum power output of your microbial fuel cells? This is not necessarily the power you get when you connect a 220Ω resistor. If you measure the voltage across several different resistors in the range of 50Ω to 5kΩ, you can generate a power-resistance curve (plotting resistance on the x-axis and calculated power on the y-axis) which allows you to determine the maximum power output of your microbial fuel cell. The resistance at which your microbial fuel cell has its maximum power output tells you what the internal resistance of your MFC is. The internal resistance which includes the resistance of the electrodes, the membrane and the MFC electrolyte, indicates how much energy is lost during electricity production. It depends on many factors such as the microbial fuel cell design, electrode spacing, the electrode size and material or the conductivity of the electrolyte (in this case your mud). How much peak power does your microbial fuel cell generate? Do you think the maximum power of your MFCs change over time?
  • What is the lifespan of the microbial fuel cells you tested in this science project? If you want to test a long-term science project, you can try this out. The MFCs may produce power for years (as long as the MFCs stays sealed and moist). But exactly how long will they keep reliably producing power? Does the power output slowly decrease over time, or does it abruptly stop? Can you see a decline long before the MFCs completely stop? Can you do something to make them have a longer lifespan?
  • The bacteria in your microbial fuel cell can only generate power if they have an organic food source they can metabolize. In this science project, the electrogenic bacteria feed on the organic compounds and nutrients that are present in the benthic mud that you collected. What if you feed your bacteria with an additional food source, such as organic waste or sugar? Will the power output change? See Science Buddies projects How Do Bacteria Produce Power in a Microbial Fuel Cell? and Powered by Pee: Using Urine in a Microbial Fuel Cell for more details.
  • Can you find a way to increase the power production of your microbial fuel cell? What could be factors that limit the power output? Think about parameters such as temperature, electrolyte conductivity, mud moisture, bacteria quantities or internal resistance. For more ideas see Science Buddies project Spice Up the Power of a Microbial Fuel Cell with a Dash of Salt.
  • Make a ground-food wastewater sample and test it to see how well it creates electricity. Caution: There is a possibility that you could make a dangerous strain of bacteria. Use protective hand, body, and face gear. Clean your work surfaces with a bleach solution and dispose of all materials in a safe manner. See the Science Buddies Project Guide page for more information about appropriate safety procedures.
  • See if you can fabricate a mediator microbial fuel cell with baker's yeast. How much electricity can it produce? How does it compare to the microbial fuel cell detailed in the Experimental Procedure? Hint: Because yeast are not very electrochemically active, you will need to use a mediator. Methylene blue is a good choice for a safe mediator.
  • Compare different microbial fuel cell designs, such as one-chambered versus two-chambered devices. Do you think the design will have an effect on their power outputs? Why? You could use one of your self-build two-chambered MFCs and compare its performance to a one-chambered MFC such as the one available from our partner Home Science Tools.


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Science Buddies Staff. "Waste Not, Want Not: Use a Microbial Fuel Cell to Create Electricity from Waste." Science Buddies, 26 Oct. 2023, https://www.sciencebuddies.org/science-fair-projects/project-ideas/Energy_p026/energy-power/use-a-microbial-fuel-cell-to-create-electricity-from-waste. Accessed 27 Feb. 2024.

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Science Buddies Staff. (2023, October 26). Waste Not, Want Not: Use a Microbial Fuel Cell to Create Electricity from Waste. Retrieved from https://www.sciencebuddies.org/science-fair-projects/project-ideas/Energy_p026/energy-power/use-a-microbial-fuel-cell-to-create-electricity-from-waste

Last edit date: 2023-10-26
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