Jump to main content

From Trash to Gas: Biomass Energy

1,153 reviews


Have you ever dreamed of a world where you could take the scraps from last night's dinner and toss them into your car's fuel tank and make gas? Well, we're not quite in "Back to the Future" yet, but in this energy science fair project, you'll discover that food scraps, dead plants, sawdust, and other decaying organic matter, called biomass are a rich source of energy. You can get energy out of biomass by burning it, turning it into a liquid, or by turning it into a gas called biogas. You've probably burned biomass (like dead wood) before if you've ever built a campfire, and you've seen biogas being produced if you've ever watched cows happily munching on green grass. The cows eat a type of biomass (grass or hay) and turn it into the biogas, methane, in their digestive tracts. In this energy science fair project, you'll compare the amounts of biogas produced by different types of biomass.


Areas of Science
Time Required
Long (2-4 weeks)
This science fair project requires access to a well-ventilated area, away from open flame or electrical sparks.
Material Availability
A small quantity of fresh cow manure is required.
Low ($20 - $50)
This science fair project produces a small amount of a flammable gas, so it must be conducted in a well-ventilated area, away from any source of open flame or source of electrical sparks. Use caution and have an adult's help when using the bleach, as it can cause chemical burns.

Kristin Strong, Science Buddies

This science fair project idea was based on an 8th grade, first-prize-winning project by A. Osegueda in 2005.


To compare the amount of biogas that is produced from different types of biomass.


It can be fun to take a drive through the beautiful countryside on a summer day, with the windows rolled down and your hair flying in the breeze. If you drive past a cattle farm though, you may be inclined to roll your windows back up! Although the smell of manure from cows and other ruminants can be strong and unpleasant, the waste is a rich source of energy that can be used to run the farm.

Manure is not the only waste that is a rich source of energy. What you might think is "just garbage," might actually be turned into energy. Dead plants, rotting food, wood chips, sawdust, leftover crops, nut shells, and paper products are all examples of biomass, which are natural materials or organic matter that can be used to make energy.

Is it hard to get energy out of biomass? No; in fact, people have been doing it for thousands of years. If you've ever sat around a campfire or fireplace, you've been warmed by burning biomass. Biomass can be burned on a larger scale to create electricity. Biomass (such as decaying plant matter, farm waste, wood waste, or industrial waste) is brought by large trucks to a huge incinerator where it is burned and the heat is used to boil water, create steam, and drive a steam generator that can produce electricity. Simply burning biomass is not great for the environment or people and animals nearby. When done on a large scale, it generates a lot of air pollution.

For this reason, scientists are interested in using green chemistry to transform biomass into liquid or gas biofuels that burn more cleanly and do not generate air pollution or contribute to climate change. The goal of green chemistry is to create useful products, like biofuels to run our cars, using methods intended to be sustainable, environmentally friendly, and do not cause harm to people. Twelve green chemistry principles cover different ways to ensure that as few resources as possible are used, and that little or no toxic waste is created. Biofuels fulfill several of these principles, including using renewable feedstocks. This means that the raw materials for the product should be replenishable. Biomass is replenishable because it comes from plant and food waste that would otherwise be thrown away.

Turning biomass into a liquid fuel involves a process called fermentation, which changes some forms of biomass—like corn, sugarcane, or switchgrass—into an alcohol-based fuel, called ethanol, which can power cars and be used as cooking fuel. Turning it into a gas is basically what cows do naturally in their digestive system, using bacteria to change biomass into methane, which is the main component in natural gas. Natural gas is an important fuel for heating homes; running stoves, ovens, and dryers; producing electricity and fertilizers; and running special cars and trucks. When made from biomass, natural gas is called biogas.

In aerobic sports—like running, walking, swimming, dancing, and bicycling—oxygen is used to generate the energy needed to continue the physical activity for a long period of time. For the creation of biogas, the opposite type of environment is needed to generate energy. Biogas is made by special anaerobic bacteria, microorganisms that live in environments without oxygen. The anaerobic bacteria break down the biomass into methane in a series of processes called anaerobic digestion. Biogas can be tapped directly from landfills,where sanitation workers dump and cover up everyone's trash, or from biogas generators where farmers or engineers mix biomass with anaerobic bacteria.

In this green chemistry science fair project, you will explore what kinds of biomass are good at making biogas. The kinds of biomass you will investigate are cow manure by itself and cow manure with vegetable peelings or with mashed banana. You will figure out how much biogas each biomass makes by filling empty soda bottles with these different types of biomass, sealing each bottle with a balloon, and measuring the inflation of the balloons (inflated mostly by methane!) over several days. Which biomass makes the most biogas?

Terms and Concepts



These sources describe what biomass is and how it can be used to make energy:

This source describes the different types of renewable energy and the role each plays in the United States:

This source describes how a California power, gas, and electric company gets auto fuel from cow manure:

Visit these pages, from PG&E, a California power, gas, and electric company, for more information about electricity:

For help creating graphs, try this website:

  • National Center for Education Statistics (n.d.). Create a Graph. Retrieved February 26, 2009.

Materials and Equipment

Disclaimer: Science Buddies participates in the Science Education Affiliate Network (SEAN). For more information, visit our Science Fair Approved Suppliers page.

Experimental Procedure

Preparing the Soda Bottles for Filling

  1. Wash and dry the soda bottles. Recycle their caps.
  2. With masking tape and a pen, label three of the bottles Cow Manure.
  3. With masking tape and a pen, label three of the bottles Cow Manure + Vegetable Peelings.
  4. With masking tape and a pen, label three of the bottles Cow Manure + Mashed Banana.
  5. Using the permanent marker and ruler, make a small horizontal mark that is 2 centimeters (cm) from the top of each bottle. This is how full you will be filling each bottle. If you try to fill the bottles to the very top, it can be easy to overflow them.
  6. Check nine balloons for any small holes that air might leak out of. If you find a balloon with a hole, replace it with one that does not have a hole.
    1. You can check if a balloon has a hole by blowing a little air into it, holding the main hole shut, and listening for air escaping and watching for the balloon to slowly deflate.

Preparing Cow Manure Bottles

  1. Put on a pair of disposable gloves.
  2. Put one of the small paper cups on the balance scale and zero out the scale (or record the weight of the cup so you can subtract it from the final weight later).
  3. Measure out 40 grams (g) of fresh cow manure in to the cup on the scale. You may want to use a disposable plastic spoon to help you scoop the manure. Carefully scoop the 40 g of cow manure (not the paper cup) in one of the soda bottles labeled Cow Manure. Throw the paper cup away.
    1. It is very important to use fresh cow manure in this project. Figure 1 shows a picture of fresh cow manure (probably less than 1 day old). Fresh manure is usually very moist, very soft, dark green/brown in coloring, and very smelly! Manure that is a few days old may still work, but for best results use manure that is as fresh as possible.
Fresh cow manure stored in a plastic bagImage Credit: Teisha Rowland, Science Buddies / Science Buddies

Figure 1. Be sure to use fresh manure for this project, as shown in a plastic bag in this picture.

  1. Repeat step 2 until all three bottles with this label have each been filled with 40 g of cow manure.

Preparing Cow Manure + Vegetable Peelings and Cow Manure + Mashed Banana Bottles

  1. Using the balance scale and a fresh small paper cup, measure out 20 g of cow manure and carefully scoop the 20 g of cow manure (not the paper cup) inside one of the soda bottles labeled Cow Manure + Vegetable Peelings. Throw the paper cup away. Repeat this step until all three bottles with this label have 20 g of cow manure inside.
  2. Using the balance scale and a fresh small paper cup, measure out 20 g of vegetable peelings, such as the peelings shown in Figure 2 below, and carefully place the 20 g of peelings inside one of the bottles labeled Cow Manure + Vegetable Peelings. Throw the paper cup away. Repeat this step until all three bottles with this label have 20 g of vegetable peelings inside.
Fresh carrot and potato peelings in a bowlImage Credit: Teisha Rowland, Science Buddies / Science Buddies

Figure 2. You can use fresh vegetable peelings from carrots and potatoes, as shown here.

  1. Repeat steps 1-2 for the Cow Manure + Mashed Banana bottles, substituting mashed banana, such as shown in Figure 3 below, for the vegetable peelings.
Thoroughly mashed banana in a bowlImage Credit: Teisha Rowland, Science Buddies / Science Buddies

Figure 3. Make sure the banana is mashed fairly well, as shown here.

  1. Take off your disposable gloves and throw them away. Wash your hands with soap and warm water, and dry them well. Put on a fresh pair of lab gloves.

Finishing the Bottles

  1. Place the funnel in one of the soda bottles.
  2. Carefully fill the bottle with distilled water to the mark you made 2 cm from the top of the bottle. Pour very slowly as you near the top so that the bottle does not overflow.
  3. Cover the mouth of the filled soda bottle with a completely uninflated balloon.
    1. When you are putting the balloon on the mouth of the bottle it is very important that you hold the balloon so that it is completely uninflated. If there is air in the balloon, when you put it on the mouth of the bottle the air will remain trapped in the balloon and this can give you false results.
    2. As you put the balloon on the mouth of the bottle, aim the balloon so that its opening is right over the mouth of the bottle (and not off to the side, where its opening might be blocked by the side of the bottle's top).
    3. Be sure to only use a balloon that you have made sure has no small holes that air might leak out of.
  4. Secure the balloon at the mouth of the soda bottle with a couple strips of heavy duty, clear shipping tape. Make sure that the entire opening of the balloon is completely sealed around the mouth of the bottle.
    1. Do not adjust the shipping tape once you have put it on the balloon because this can rip the balloon.
  5. Repeat steps 1-4 until all soda bottles have been filled and topped with balloons. When you are done, you should have bottles that look like the ones in Figure 4 below (except three bottles for each condition, not just one).
Uninflated balloons are taped over the mouths of three plastic bottles filled with a mix of water and cow manureImage Credit: Teisha Rowland, Science Buddies / Science Buddies

Three plastic bottles are filled with water and fresh cow manure. Vegetable peelings are also added to one bottle and a mashed banana is added to another. A deflated balloon seals the top of each bottle and is secured with tape. The bottles are then left in a well ventilated area.

Figure 4. When you are done assembling your bottles, they should look like the ones in this picture.

  1. Take off your lab gloves and throw them away. Wash your hands with soap and warm water. Because you were working with waste, it is important to thoroughly clean the scale, funnel, and work area with a bleach solution. Review the Science Buddies Microorganisms Safety Guide for more information.

Testing the Bottles

  1. Place the soda bottles in a well-ventilated area, away from open flame or sparks of electricity, such as outdoors on a porch or balcony.
    1. If the bottles are in a relatively cold place, it may take longer for the balloons to inflate than if the bottles were placed in a warmer place.
  2. Use the measuring tape to measure the circumference (in centimeters) of each balloon, at its fullest part. Record the date, time, and measurements in a data table in your lab notebook.
  3. Each day, at approximately the same time, repeat step 2 for a total of 12 days.
    1. It may take up to about a week before you can visibly see some of the balloons inflating. If you think that the balloons are not inflating as they should, see the "Troubleshooting" section below.
  4. When you measure the balloons, take a look at the bottles too. You may notice that in some bottles a large amount of biomass has floated to the top of the bottle, as shown in Figure 5 below. If this happens, you should make a note in your lab notebook and carefully tilt the bottle by about 45 degrees (so that it is momentarily horizontal) to dislodge the biomass. When you tilt the bottle, make sure that the balloon is securely on the mouth of the bottle and that no other leaks may allow liquid to escape.
    1. If enough biomass accumulates at the top of the bottle, it may prevent some biogas from getting into the balloon.
    2. If you need to tilt one bottle, you should tilt all of the other bottles. It is important that all of the bottles are treated the same way.
    3. You may notice a large amount of biomass floating at the top of some bottles every day during your experiment. Do not worry if this happens. Simply carefully tilt all of the bottles every time you see this, as described above.
Biomass build up in the neck of a plastic bottle traps gas from rising into the balloon attached to the topImage Credit: Teisha Rowland, Science Buddies / Science Buddies

Figure 5. If you see a buildup of biomass at the top of any of your bottles, as shown here, this can prevent some biogas from getting into the balloon. To dislodge the biomass, carefully tilt the bottle (so that it is momentarily horizontal). Make sure you treat all of the bottles the same way.

Analyzing Your Data Table

  1. For the bottles labeled Cow Manure, make a graph that plots the circumferences of the balloons on the y-axis (in centimeters) and the day number (1-12) on the x-axis. You can make the graph by hand or use a website like Create a Graph to make the graph on a computer and print it.
  2. For the bottles labeled Cow Manure + Vegetable Peelings, make a graph that plots the circumferences of the balloons on the y-axis and the day number (1-12) on the x-axis.
  3. For the bottles labeled Cow Manure + Mashed Banana, plot the circumferences of the balloons on the y-axis and the day number (1-12) on the x-axis.
  4. Comparing the three graphs, which type of biomass produced the fastest inflation of the balloons? Which type of biomass produced the greatest inflation of the balloons? Overall, which biomass do you think is the best at making biogas? What do you think it is about the best performing biomass that makes it so good at making biogas?


  1. Do not worry if not all of your balloons become inflated. Not all bottles may produce enough biogas to inflate the balloons, but you should see the balloons become inflated by at least some of the bottles. If none of your balloons become inflated, here are some factors you can try changing for better results:
    1. Make sure you are using fresh manure. See step 3a in "Preparing Cow Manure Bottles" above for details.
    2. You could try increasing the amount of manure you use. If you do this, make sure you increase the amount by the same amount for every condition, and that you increase the amount of vegetable peels and mashed banana by the same amount as well.
    3. Check to make sure that the balloons do not have any tiny holes and that they are tightly secured on the mouth of the bottle. You can do this by holding the balloon gently in one hand, and gently and slowly squeezing the bottle a little with your other hand. You should feel the balloon inflate a little.
  2. If the three bottles for the same condition (for example, all the bottles with cow manure and mashed bananas) do not seem very consistent in how much biogas they are producing, this could be because different amounts of air were trapped in the balloons when they were placed on the bottles. See step 3a in "Finishing the Bottles" above for details.
  3. Some of the inflated balloons may deflate a little bit (by 2 cm or so) after about a week. Do not worry if this happens, as a tiny amount of biogas may escape from the inflated balloons over time. However, if an inflated balloon deflates a lot, this may be a sign of a leak in the balloon.
icon scientific method

Ask an Expert

Do you have specific questions about your science project? Our team of volunteer scientists can help. Our Experts won't do the work for you, but they will make suggestions, offer guidance, and help you troubleshoot.

Global Connections

The United Nations Sustainable Development Goals (UNSDGs) are a blueprint to achieve a better and more sustainable future for all.

This project explores topics key to Affordable and Clean Energy: Ensure access to affordable, reliable, sustainable and modern energy.
This project explores topics key to Responsible Consumption and Production: Ensure sustainable consumption and production patterns.


  • Investigate how the following affect biogas production:
    1. Temperature
    2. pH
    3. Antibiotics
    4. Detergent
  • Using the resources in the Bibliography, above, and with the help of an adult, devise a safe way to test whether the biogas you produced is flammable.
  • Biogas production can be increased by delignification (removing lignin) in the biomass before putting it into the biogas generator. One way to achieve delignification of a biomass is through white rot fungus. Compare the biogas production of the same biomass with and without an initial treatment of white rot fungus.
  • Add negative controls to the main experiment above by including six additional test bottles. Fill three of the bottles with 20 g of mashed banana only (add no manure), and three of the bottles with 20 g of vegetable peelings only (add no manure). Add distilled water to the bottles, then seal and test them with the other nine bottles, as directed above. How do your negative controls compare to the mashed banana and vegetable peelings test bottles that do contain cow manure? What happened with the addition of the cow manure? What component of the cow manure do you think accounts for the differences?


If you like this project, you might enjoy exploring these related careers:

Career Profile
Every day, trash collectors across the nation haul our garbage (minus the recyclables) to a landfill. There, the garbage decomposes into gas and leachate. This sounds gross, but the good news is that the gas can be used to create electricity. Landfill gas-collection power plants harvest the methane gas from landfills, then burn it to generate electricity. These power plants need people to keep the equipment functioning properly. Methane gas generation systems technicians are responsible for… Read more
Career Profile
Chemical engineers solve the problems that affect our everyday lives by applying the principles of chemistry. If you enjoy working in a chemistry laboratory and are interested in developing useful products for people, then a career as a chemical engineer might be in your future. Read more
Career Profile
Soybeans, corn, grass, algae, vegetable oil: What do these things have in common? The answer is that they can be used to create biofuels, which are derived from plants or plant material. These sources are called biomass. The procedure for converting plants or vegetable oil into a biofuel is a chemical reaction that has many steps. The biofuel or biodiesel technology and product development manager is a professional who develops ways to convert the raw material into fuel and improve the types,… Read more
Career Profile
Are you passionate about the environment? Do you like developing and implementing new ideas? Do you enjoy talking with people about how humans impact nature? If these things are true about you, then you may be the ideal candidate for a job as a sustainability specialist. Sustainability specialists work in large and small corporations and universities to design and execute energy and resource conservation programs that reduce their employers' impact on the environment. This is a great career for… Read more

News Feed on This Topic

, ,

Cite This Page

General citation information is provided here. Be sure to check the formatting, including capitalization, for the method you are using and update your citation, as needed.

MLA Style

Science Buddies Staff. "From Trash to Gas: Biomass Energy." Science Buddies, 30 Mar. 2022, https://www.sciencebuddies.org/science-fair-projects/project-ideas/Energy_p027/energy-power/from-trash-to-gas-biomass-energy. Accessed 21 Apr. 2024.

APA Style

Science Buddies Staff. (2022, March 30). From Trash to Gas: Biomass Energy. Retrieved from https://www.sciencebuddies.org/science-fair-projects/project-ideas/Energy_p027/energy-power/from-trash-to-gas-biomass-energy

Last edit date: 2022-03-30
Free science fair projects.