Hello,
I did not perform a single 1:10 dilution while testing the invertase activity in procedure two.
Help with glucose project
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evanthebioguy
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Re: Help with glucose project
Hello,
It looks like you did the calculations correctly based on the data that you got. In this case, I'm guessing that the invertase enzyme wasn't converting sucrose to glucose at the same rate in the 10% sucrose solution as it was in the food you tested. Did you test the sucrose solution and the foods in the same day? Any differences between your experimental setup in part 2 and part 3 of the procedure could give you results that don't match what you expected. Did you let the invertase enzyme sit out at room temperature for a long time before using it? That could decrease its activity and affect your results. Did you make sure that the water and all of the foods and sucrose solution that you tested were at room temperature before you started using them? Differences in temperature have a big influence on enzyme activity, so that could also cause variability in your results. Since you tested 15 mL of the sucrose solution, did you test 15 mL of the foods as well? Think about anything that might have been different in the way you tested the 10% sucrose solution and the foods. You could definitely try the experiment again and see if you get more consistent results this time. If you don't have time to do the experiment again, I think it's okay to just report the results that you got and comment on why you think your results might not have come out as expected. It's very common for science experiments to not work out the way that you expected on the first try. A big part of science is learning how to troubleshoot and modify your experiments based on the new information that you learn along the way.
It looks like you did the calculations correctly based on the data that you got. In this case, I'm guessing that the invertase enzyme wasn't converting sucrose to glucose at the same rate in the 10% sucrose solution as it was in the food you tested. Did you test the sucrose solution and the foods in the same day? Any differences between your experimental setup in part 2 and part 3 of the procedure could give you results that don't match what you expected. Did you let the invertase enzyme sit out at room temperature for a long time before using it? That could decrease its activity and affect your results. Did you make sure that the water and all of the foods and sucrose solution that you tested were at room temperature before you started using them? Differences in temperature have a big influence on enzyme activity, so that could also cause variability in your results. Since you tested 15 mL of the sucrose solution, did you test 15 mL of the foods as well? Think about anything that might have been different in the way you tested the 10% sucrose solution and the foods. You could definitely try the experiment again and see if you get more consistent results this time. If you don't have time to do the experiment again, I think it's okay to just report the results that you got and comment on why you think your results might not have come out as expected. It's very common for science experiments to not work out the way that you expected on the first try. A big part of science is learning how to troubleshoot and modify your experiments based on the new information that you learn along the way.