I'm doing a project about influenza viruses. The experiment shows the effectiveness of different vaccines. In order to find out the effect I have to use the a program called the BLAST. Unfortunately I don't know how to work the BLAST program.
To read the experiment:
https://www.sciencebuddies.org/mentorin ... l?from=TSW
The procedures of the experiment are listed under the subtitle:
Experimental Procedure
Please answer!
Thank you.
Influenza Viruses-BLAST
Moderators: AmyCowen, kgudger, bfinio, MadelineB, Moderators
Re: Influenza Viruses-BLAST
Hi there,
If you want to use BLAST directly on the ISD website, it looks like you would need to purchase a subscription. This would be the easiest option since it narrows your search down to virus sequences only, as opposed to sequences from all types of organisms. However, it costs a lot of money.
The alternative is to get the sequence data from ISD, then use the general BLAST tool available at NCBI. Here is a tutorial on using the NCBI BLAST:
http://www.ncbi.nlm.nih.gov/Education/B ... orial.html
Since you want to look at the DNA sequence, not the protein sequence, you will want to use "blastn" (the nucleotide blast).
Hope this helps,
Grace
If you want to use BLAST directly on the ISD website, it looks like you would need to purchase a subscription. This would be the easiest option since it narrows your search down to virus sequences only, as opposed to sequences from all types of organisms. However, it costs a lot of money.
The alternative is to get the sequence data from ISD, then use the general BLAST tool available at NCBI. Here is a tutorial on using the NCBI BLAST:
http://www.ncbi.nlm.nih.gov/Education/B ... orial.html
Since you want to look at the DNA sequence, not the protein sequence, you will want to use "blastn" (the nucleotide blast).
Hope this helps,
Grace