Hello! I have a science fair project that I came up with. I need some help with growing the bacteria. So my project question is:
How does light affect methanotrophic culture?
Obviously, I need to grow the methanotrophs first. However, I am not sure how to do this because I need to incubate them in CH4 and isolate them.
Please help!!!
My Science Fair Project
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Re: My Science Fair Project
Hi! This sounds like a really interesting project!
Can you tell me a bit more about what your current setup plan is is, and what your concerns about incubating them in CH4 is? We can work up from there!
-Rebekah
Can you tell me a bit more about what your current setup plan is is, and what your concerns about incubating them in CH4 is? We can work up from there!
-Rebekah
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- Posts: 2
- Joined: Mon Sep 12, 2022 5:06 pm
- Occupation: Student
Re: My Science Fair Project
Hello! Thank you so much for answering!
I want to incubate methanotrophs but first I have to extract their pure culture. I have to perform a pre-incubation of soil (or my methanotroph source) with CH4 and then a serial dilution in order to obtain their pure cultures. Then, I need to incubate the petri dishes with the diluted sample with CH4 again. However, I am not sure how to obtain the CH4 and incubate my bacteria without an incubator.
I want to incubate methanotrophs but first I have to extract their pure culture. I have to perform a pre-incubation of soil (or my methanotroph source) with CH4 and then a serial dilution in order to obtain their pure cultures. Then, I need to incubate the petri dishes with the diluted sample with CH4 again. However, I am not sure how to obtain the CH4 and incubate my bacteria without an incubator.