5' and 3' in DNA sequences
5' and 3' in DNA sequences
How can you tell which end of a DNA sequence is the 5' end?
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- Former Expert
- Posts: 393
- Joined: Tue Sep 20, 2005 2:40 pm
Conventionally sequences are written down starting with the 5' end so if you are looking at the sequence from a database or journal article it is most likely written 5' to 3'. If it is written in reverse it would probably be annotated as such.
If you tell us a little more about the sequence we can help you be sure.
Best of luck,
Caroline
If you tell us a little more about the sequence we can help you be sure.
Best of luck,
Caroline
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- Former Expert
- Posts: 26
- Joined: Tue Oct 03, 2006 12:04 pm
End Descriptions
If you look at the site:
http://web.mit.edu/esgbio/www/dogma/repl.html
You'll find that the 3' is the OH end of a strand and the 5' is the Phosphorous end-- at least that's how I read it.
Good luck.
http://web.mit.edu/esgbio/www/dogma/repl.html
You'll find that the 3' is the OH end of a strand and the 5' is the Phosphorous end-- at least that's how I read it.
Good luck.
Hope this helps,
-Bob-
-Bob-