Measuring Enzyme Activity: Yeast Catalase *
|Time Required||Average (6-10 days)|
|Safety||Adult supervision is recommended for this project.|
AbstractYeast contains an enzyme, called catalase, that acts as a catalyst for the reaction that breaks down hydrogen peroxide into oxygen and water (2H2O22H2O + O2). Safety note: oxygen is a highly reactive gas, adult supervision recommended for this project. For your background research, be sure that you understand substrate, catalyst, reaction rate, catalase, enzyme saturation and protein denaturation. Use a solution of 3% H2O2 for the substrate. Construct an apparatus that allows you to collect and measure the oxygen gas produced (for a description, see: An Aerobic Exercise: Yeast Metabolism with and without Aeration). Here are some questions you might try to answer with your project: What evidence do you have that the gas you are collecting is oxygen? How does the reaction rate depend on the amount of substrate? At what substrate concentration does the reaction show evidence of enzyme saturation? How does the reaction rate depend on temperature (try increments of 10°C)? At what temperature does the reaction show evidence of protein denaturation? How does the activity of yeast catalase compare to catalase from potato extract or liver extract? How does it compare to the activity of iron oxide? (Gardner, 1999, 97-99; Hamamah, 2005)
Cite This PageGeneral citation information is provided here. Be sure to check the formatting, including capitalization, for the method you are using and update your citation, as needed.
Last edit date: 2017-07-28
- Gardner, R., 1999. Science Projects About Kitchen Chemistry. Berkeley Heights, NJ: Enslow Publishers.
- Hamamah, A. A., 2005. "How Do Substrate Amount, Temperature, pH, Enzyme Amount, and Inhibitor Affect Catalase Activity?" California State Science Fair 2005 Project Summary [accessed January 18, 2006] http://www.usc.edu/CSSF/Current/Projects/J0406.pdf.
News Feed on This Topic
Ask an ExpertThe Ask an Expert Forum is intended to be a place where students can go to find answers to science questions that they have been unable to find using other resources. If you have specific questions about your science fair project or science fair, our team of volunteer scientists can help. Our Experts won't do the work for you, but they will make suggestions, offer guidance, and help you troubleshoot.
Ask an Expert
If you like this project, you might enjoy exploring these related careers:
BiochemistGrowing, aging, digesting—all of these are examples of chemical processes performed by living organisms. Biochemists study how these types of chemical actions happen in cells and tissues, and monitor what effects new substances, like food additives and medicines, have on living organisms. Read more
Commercial & Industrial DesignerHave you always loved art? Do you have a good eye for beauty, balance, and form? How would you like to see your designs show up in toy stores? Or in a sporting goods store? Or at a car dealer? Commercial and industrial designers create the shape and form of every type of manufactured good that you can think of—from toys, sporting goods, and medical equipment to high technology products, furniture, toothbrushes, and toasters. They design the form of new products that are as beautiful and pleasing to look at as they are functional. Read more
News Feed on This Topic
Looking for more science fun?
Try one of our science activities for quick, anytime science explorations. The perfect thing to liven up a rainy day, school vacation, or moment of boredom.Find an Activity