Abstract
Plants need nitrogen to grow healthy stems and leaves. Although nitrogen is the most abundant element in the air we breathe, that form of nitrogen cannot be used by plants. Nitrogen contained in fertilizer, on the other hand, is readily taken up by plants. In this experiment, you will compare plants grown without nitrogen fertilizer to plants grown with nitrogen fertilizer.Summary
Edited by Sabine De Brabandere, PhD, Science Buddies

Objective
The goal of this experiment is to compare the growth of plants with and without added nitrogen fertilizer.Introduction
Plants require more than just water and sunlight to grow. They also require many nutrients found in the soil. One of the most important nutrients required for plant growth is nitrogen. Nitrogen is used to build plant proteins and nucleic acids, including DNA.
Nitrogen is found naturally in the atmosphere and in the soil. Even though there is an abundance of nitrogen available, the most common form of nitrogen (N2) cannot be used by plants. Nitrogen can be combined chemically with oxygen or hydrogen to form types of nitrogen compounds that plants can use. These nitrogen compounds can be added to the soil in the form of ammonium (NH4+) and nitrate (NO3-) fertilizers. Plants grow well when fertilizer containing nitrogen is added to the soil, but this method can be expensive and has to be repeated each time the nitrogen in the soil is used up.

Figure 1. Adding fertilizers containing nitrogen to the soil can help plants grow well.
In this experiment, you will compare plants grown without nitrogen fertilizer to plants grown with nitrogen fertilizer. You will observe the effects of nitrogen on the health of the plants by measuring the increase in biomass (the total mass, or weight, of each plant) during the experiment.
Terms and Concepts
- Nutrient
- Fertilizer
- Biomass
Questions
- What nutrients do plants need to survive?
- How do plants use nitrogen and what do they use nitrogen for?
Bibliography
- For information about plant growth, check out this resource:
Easy Science For Kids (n.d.). Photosynthesis: How Plants Make Food and Energy? Retrieved December 15, 2015, from http://easyscienceforkids.com/all-about-photosynthesis - This website provides information for young gardeners:
University of Illinois Extension. (2015). My First Garden. Retrieved December 15, 2015, from http://extension.illinois.edu/firstgarden/basics/index.cfm
Materials and Equipment
Note: Adult supervision recommended during preparation of nitrogen fertilizer solution.- At least eight small pots
- Potting soil
- One packet of seeds
- Commercial nitrogen fertilizer mix for house plants (e.g., Miracle Gro, Peter's or equivalent)
- Empty glass or plastic bottle for mixing nitrogen fertilizer
Experimental Procedure
- Make sure pots have holes in the bottom to allow the roots to "breathe" and excess water to drain out.
- Measure equal amounts of soil into each of the pots.
- Moisten the soil in each pot with the equal amounts of water.
- Label four pots with "nitrogen fertilizer" and four pots with "no nitrogen added."
- Plant seeds in each pot according to seed packet instructions.
- Place plants near a sunny window or under a grow light.
- Check your plants regularly and water when needed.
- Soil should be kept moist.
- Water the "no nitrogen added" pots with regular water.
- Water the "nitrogen fertilizer" pots with water mixed with nitrogen fertilizer. Follow the fertilizer manufacturer's recommendations for preparing this solution.
- All plants should receive the same amount of liquid at each watering.
- Keep track of your watering schedule in your lab notebook.
- At regular intervals, record observations on the growth of the plants (e.g., height, number of leaves on each plant, etc.) in your lab notebook. For information on scientifically measuring plant growth see the Science Buddies How-to page Measuring Plant Growth.
- Here is a sample data table for organizing your data:
No Fertilizer | With Fertilizer | |||||||
---|---|---|---|---|---|---|---|---|
#1 | #2 | #3 | #4 | #5 | #6 | #7 | #8 | |
Date:
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Date:
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Date:
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Etcetera |
- Allow plants to grow to maturity, then make final measurements of the growth of each plant. The following resource page provides some suggested methods for accurately quantifying plant growth: Measuring Plant Growth.
- Summarize your results by averaging the "No Fertilizer" and "With Fertilizer" conditions, and prepare one or more bar graphs to compare your measurements. The resource page Data Analysis & Graphs can help you.
- Summarize your results or draw conclusions based on your data. Check out the resource page Conclusions if you need help.
- Make an attractive Display Board and share your results.

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Variations
- Compare different amounts of nitrogen fertilizer to see what the ideal concentration is for growing plants.
- Experiment with other types of nutrient fertilizers to test their effects on plants.
- Compare different soil types on germination and young plant growth.
- The following project might interest you as well: Earthworm Castings — The Ideal Proportion in Soil for Young Garden Plants
Careers
If you like this project, you might enjoy exploring these related careers:
Related Links
- Science Fair Project Guide
- Other Ideas Like This
- Plant Biology Project Ideas
- My Favorites
- Measuring Plant Growth