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Polymer Polymer Polymer Elemental and Chemical Analysis The material, chemical structure, chemical composition and physicochemical properties can significantly impact the polymer's function. For example, polymeric functional membrane materials require the polymer to have a microporous structure or a diffusion function  to meet the selective permeation requirement of the separated material in the membrane. The reactive functional polymer needs to have a certain swelling property or a range of… Read more
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CRISPR CRISPR CRISPR Cas9 Gene Editing for Target Identification and Validation CRISPR Cas9 gene editing system consists of a Cas9 protein (or other homologous protein) and a single guide RNA (gRNA). The function of the CRISPR system involves RNA-guided cleavage of the exogenous double-stranded DNA, followed by imprecise repair through either imprecise non-homologous end joining (NHEJ) or template-dependent homologous directed repair (HDR) pathways. CRISPR Cas9 works just like a pair of… Read more
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Fluorescence Application Fluorescence Application Fluorescence Labeling of Peptides Fluorescent labels or probes are ideal for many imaging and diagnostic applications. Fluorescent groups can be covalently connected to the N-terminal or C-terminal, although it is recommended to bind to the N-terminal. FRET pairs composed of receptors and quenching agents can be connected internally or externally. For longer sequences, it is recommended that you connect FRET pairs internally. Fluorescent… Read more
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drug delivery system drug delivery system PEGylation of Liposomes Liposome is an ideal drug delivery carrier. It has targeted properties, longer blood retention time and higher organ distribution selectivity, can improve the efficacy of drugs and reduce toxic side effects. Ordinary liposomes have the disadvantage of being easily cleared from the systemic circulation by liver and spleen macrophages. In this case, PEGylation can solve the above shortcomings, making liposomes stay in the blood… Read more
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biochemistry biochemistry NHS Esters NHS esterrs are the most commonly used amine reactive reagents. Under physiological pH or even weakly alkaline conditions, NHS esters can react with primary amines to form very stable amide bonds. Both the N-terminal α-amino group and the lysine residue ε-amino group of the protein can form an amide bond with the NHS ester. The amide bond formed by NHS ester and amino group is exactly the same as the peptide bond in natural protein. NHS esters are… Read more
Ask an Expert Topic Forum: Grades 9-12: Physical Science
Electro Culture Electro Culture Hello, I'm planning on conducting an experiment regarding electro culture for a few crops. My only issue is that the articles I've seen regarding the topic fail to mention how to go about creating a "mild electric current" for the crops. I'm wondering if anyone here can provide some information on how to set up such a current and what is meant by "mild." Is there a particular number of volts that can be classified as "mild" in this context? Much thanks. Read more
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Ask an Expert Topic Forum: Grades 6-8: Physical Science
Question about Build a Paper Rocket activity Question about Build a Paper Rocket activity Type your question below and then press Submit! Hi there, I tried to change in my paper rocket's design by increasing the fins... At the begining I added to boosters and watched the performence then I tried to add more fins but nothing was happened.... I expected more time would be consumed to the rocket to reach the same point as a result of resisting gravity because of the fins but I did not Notice… Read more
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Ask an Expert Topic Forum: Grades K-5: Physical Science
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