Ninth Grade, Biotechnology Projects, Lessons, Activities (Page 2)

CRISPR Gene Editing of Escherichia coli

Science Fair Project Idea

Scientific Method

Many scientists are currently very excited about CRISPR, as it has the potential to revolutionize gene editing. But what exactly is CRISPR and what does it do? CRISPR is a novel tool in gene editing that allows the modification of genetic DNA at specific target sites in many different organisms. Researchers have high hopes that this technology can, one day, cure genetic diseases, as mutated DNA sequences can easily be corrected. In this project, you will use CRISPR to mutate a DNA sequence… Read more

Expression Cloning

Science Fair Project Idea

Scientific Method

What is expression cloning and how is it used in the biotechnology industry? How are plasmids constructed for use in a bacterial expression system? You can investigate these questions and more using bacterial expression kits meant for high school classes. You will need a laboratory space equipped to grow bacteria and carry out simple molecular biology. A kit with plasmids and bacterial cells to transform are also needed. Here are two possibilities: BioBuilder What a Colorful World Kit … Read more

Genetically Engineer Yeast to be Fluorescent

Science Fair Project Idea

Scientific Method

Can you imagine a glowing loaf of bread? You might not be able to make the whole loaf glow, but you can get baker's yeast to fluoresce! The way to do this is to modify the genetic information of the yeast organism. The technology that is used to do this is called genetic engineering. With genetic engineering, you can insert a fluorescent protein gene from a jellyfish into yeast cells, so they start glowing under blue light! Do this project to see for yourself! Read more

CUT! How Does CRISPR Work?

Lesson Plan Grade: 6th-12th

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In this lesson plan, students will take a closer look at the most recent developments in gene editing. Specifically, they will learn about the CRISPR technology using various interactive simulations and other resources. Based on their gained knowledge, students will create a model of the CRISPR-Cas9 components and create a stop-motion animation video of the molecular mechanism of CRISPR-Cas9. Remote learning adaptation: This lesson plan can be conducted remotely. Students can work… Read more

NGSS Performance Expectations:

MS-LS4-5. Gather and synthesize information about technologies that have changed the way humans influence the inheritance of desired traits in organisms.
HS-LS1-1. Construct an explanation, based on evidence, for how the structure of DNA determines the structure of proteins, which carry out the essential functions of life through systems of specialized cells.

Protein Fingerprinting

Science Fair Project Idea

Scientific Method

First came the Human Genome Project and now (drum roll please) the Human Proteome Project. Confused? Not surprising as the Human Proteome Project has not received the kind of press that the Human Genome Project did. Nonetheless it is a major, and potentially important, scientific undertaking. Just as the genome is the complete set of an organism's DNA, the proteome is all the proteins expressed in an organism. Why study the proteome? It is because proteins are the work horses of biological… Read more

Design 'Y'er Genes

Lesson Plan Grade: 9th-12th

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This lesson introduces students to the relationships between chromosomes, genes, and DNA molecules. Using the example of a strawberry, it also provides activities that clearly show how changes in the DNA of an organism, either naturally or artificially, can cause changes. Read more

NGSS Performance Expectations:

HS-LS3-3. Heredity: Inheritance and Variation of Traits
Apply concepts of statistics and probability to explain the variation and distribution of expressed traits in a population.

From Prokaryotes to Eukaryotes: Solving Problems Using Plasmids

Lesson Plan Grade: 9th-12th

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This lesson compares and contrasts prokaryotic and eukaryotic cells and examines the form and function of the plasmid found in prokaryotic cells. Students will then use these principles to simulate how a desirable gene can be isolated and inserted into a plasmid as one step in the process of creating a genetically modified organism (GMO). Read more

NGSS Performance Expectations:

HS-LS1-2. Develop and use a model to illustrate the hierarchical organization of interacting systems that provide specific functions within multicellular organisms.

Purification Strategies

Science Fair Project Idea

Scientific Method

In order to study individual biochemical compounds like proteins, DNA, or RNA, biochemists need to know how to purify these components from a complex mixture. This is especially important for biotechnology and pharmaceutical industries, which sell purified biochemicals as reagents or drugs to consumers. Do an experiment to purify DNA, RNA, or protein from a complex mixture (for purifying DNA, see the Science Buddies project Extracting Onion DNA). The source of the material can be a cell… Read more

Solubility of Proteins

Science Fair Project Idea

Scientific Method

Some proteins are soluble in aqueous solutions and some are not. Insoluble proteins can be a problem because the proteins can form large aggregates in solution which are difficult to purify, crystallize, and use in experiments. Compare the amino acid sequence and 3-D structures of some soluble and insoluble proteins (Berman, 2000). Is the structure polar or non-polar, and how might this affect solubility? How do detergents make an insoluble protein more soluble? Investigate how this… Read more

The Making of a GMO Crop

Lesson Plan Grade: 9th-12th

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Students will compare and contrast methods of selective plant breeding, describe the scientific process of creating a genetically modified plant, compare genetically modified soybean seeds to conventional soybean seeds, describe the impact weeds have on plant growth, and understand how a genetically modified seed can help farmers manage weeds. Read more

NGSS Performance Expectations:

HS-LS1-1. Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins, which carry out the essential functions of life through systems of specialized cells.
HS-LS3-1. Ask questions to clarify relationships about the role of DNA and chromosomes in coding the instructions for characteristic traits passed from parents to offspring.

Ninth Grade, Biotechnology Projects, Lessons, Activities (22 results)

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